Stanford/BIOE44:Module 2

Module 2: Genetic Devices

Inventory for Arsenic Sensor

Group Members	Part	Status	Volume	Concentration
Daniel and Aaditya	arsR insert	Digested at E and S	25 uL	36.8 ng/uL
	arsR insert	Digested at X and S, extracted from gel	2 gel bands	(hasn't been purified yet)
Travis and Scott	none
Frank, Claire, and Sanjay	pSB2k3 vector (with K274111 insert)	undigested	a substantial amount	203 ng/ul
	color 004 light green insert	digested X,P and running in gels	~15uL	110 ng/uL
	arsR insert	digested E,S and running in gels	~15 uL	133 ng/uL
	color 004 light green insert	digested X,P and not running in gels yet	40 uL	110 ng/uL
	arsR	digested E,S and not running in gels yet	40 uL	133 ng/uL
	MG1655 vector (low copy vector and red-orange insert)	digested with E,P (for double ligation) and X,P (for single ligation); not running in gels yet	25uL each	273 ng/uL

Sunny, Alex | pSB2K3 vector (with K274111 insert) | undigested | 48 uL | 8.8 ng/ul |- | | color 002 purple insert | digested X,P, gel extracted | | 0 ng/uL (no DNA retrieved from gels) |- | | arsR insert | digested E,S | | 11.1 ng/uL |- | | S03511 vector (low-medium copy #, inducible) | undigested | 48uL | 41.2 ng/uL |- | | I3023 vector (low-medium copy #, inducible) | undigested | 48uL | 97.2 ng/uL |- | | I3023 vector (low-medium copy #, inducible) | undigested | 48uL | 93.7 ng/uL |- | I3002 vector (low-medium copy #, constitutive) | undigested | 48uL | 103.9 ng/uL- | | - | I3002 vector (low-medium copy #, constitutive) | undigested | 48uL | 109.1 ng/uL- |

Introduction

TBA

Schedule

TBA