Stanford/BIOE44:Module 2: Difference between revisions
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| Line 65: | Line 65: | ||
| 25uL each | | 25uL each | ||
| 273 ng/uL | | 273 ng/uL | ||
|- | |||
Sunny, Alex | | | ||
|Sunny, Alex | |||
| pSB2K3 vector (with K274111 insert) | | pSB2K3 vector (with K274111 insert) | ||
| undigested | | undigested | ||
Revision as of 16:09, 22 April 2010
Module 2: Genetic Devices
Inventory for Arsenic Sensor
| Group Members | Part | Status | Volume | Concentration | |
| Daniel and Aaditya | arsR insert | Digested at E and S | 25 uL | 36.8 ng/uL | |
| arsR insert | Digested at X and S, extracted from gel | 2 gel bands | (hasn't been purified yet) | ||
| Travis and Scott | none | ||||
| Frank, Claire, and Sanjay | pSB2k3 vector (with K274111 insert) | undigested | a substantial amount | 203 ng/ul | |
| color 004 light green insert | digested X,P and running in gels | ~15uL | 110 ng/uL | ||
| arsR insert | digested E,S and running in gels | ~15 uL | 133 ng/uL | ||
| color 004 light green insert | digested X,P and not running in gels yet | 40 uL | 110 ng/uL | ||
| arsR | digested E,S and not running in gels yet | 40 uL | 133 ng/uL | ||
| MG1655 vector (low copy vector and red-orange insert) | digested with E,P (for double ligation) and X,P (for single ligation); not running in gels yet | 25uL each | 273 ng/uL | ||
| Sunny, Alex | pSB2K3 vector (with K274111 insert) | undigested | 48 uL | 8.8 ng/ul | |
| color 002 purple insert | digested X,P, gel extracted | 0 ng/uL (no DNA retrieved from gels) | |||
| arsR insert | digested E,S | 11.1 ng/uL | |||
| S03511 vector (low-medium copy #, inducible) | undigested | 48uL | 41.2 ng/uL | ||
| I3023 vector (low-medium copy #, inducible) | undigested | 48uL | 97.2 ng/uL | ||
| I3023 vector (low-medium copy #, inducible) | undigested | 48uL | 93.7 ng/uL | ||
| I3002 vector (low-medium copy #, constitutive) | undigested | 48uL | 103.9 ng/uL | ||
| I3002 vector (low-medium copy #, constitutive) | undigested | 48uL | 109.1 ng/uL |
{Sunny, Alex | pSB2K3 vector (with K274111 insert) | undigested | 48 uL | 8.8 ng/ul |- | | color 002 purple insert | digested X,P, gel extracted | | 0 ng/uL (no DNA retrieved from gels) |- | | arsR insert | digested E,S | | 11.1 ng/uL |- | | S03511 vector (low-medium copy #, inducible) | undigested | 48uL | 41.2 ng/uL |- | | I3023 vector (low-medium copy #, inducible) | undigested | 48uL | 97.2 ng/uL |- | | I3023 vector (low-medium copy #, inducible) | undigested | 48uL | 93.7 ng/uL |- | I3002 vector (low-medium copy #, constitutive) | undigested | 48uL | 103.9 ng/uL- | | - | I3002 vector (low-medium copy #, constitutive) | undigested | 48uL | 109.1 ng/uL- |}
Introduction
TBA
Schedule
TBA
