Stanford/BIOE44:Module 2: Difference between revisions
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==Inventory for Arsenic Sensor== | ==Inventory for Arsenic Sensor== | ||
{| style="width: | {| style="width:95%" border=1px | ||
|'''Group Members''' | |'''Group Members''' | ||
|'''Part''' | |'''Part''' | ||
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|'''Volume''' | |'''Volume''' | ||
|'''Concentration''' | |'''Concentration''' | ||
|- | |||
| Chris and Karina | |||
| pSB2k3 (digested (E and P), extracted, purified) | |||
| Ready for Ligation | |||
| 25 uL | |||
| 80 ng/uL | |||
|- | |- | ||
| Daniel and Aaditya | | Daniel and Aaditya | ||
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| 109.1 ng/uL | | 109.1 ng/uL | ||
|- | |- | ||
|} | |} | ||
Revision as of 23:05, 22 April 2010
Module 2: Genetic Devices
Inventory for Arsenic Sensor
Group Members | Part | Status | Volume | Concentration |
Chris and Karina | pSB2k3 (digested (E and P), extracted, purified) | Ready for Ligation | 25 uL | 80 ng/uL |
Daniel and Aaditya | arsR insert | Digested at E and S | 25 uL | 36.8 ng/uL |
arsR insert | Digested at X and S, extracted from gel | 2 gel bands | (hasn't been purified yet) | |
Travis and Scott | none | |||
Frank, Claire, and Sanjay | pSB2k3 vector (with K274111 insert) | undigested | a substantial amount | 203 ng/ul |
color 004 light green insert | digested X,P and running in gels | ~15uL | 110 ng/uL | |
arsR insert | digested E,S and running in gels | ~15 uL | 133 ng/uL | |
color 004 light green insert | digested X,P and not running in gels yet | 40 uL | 110 ng/uL | |
arsR | digested E,S and not running in gels yet | 40 uL | 133 ng/uL | |
MG1655 vector (low copy vector and red-orange insert) | digested with E,P (for double ligation) and X,P (for single ligation); not running in gels yet | 25uL each | 273 ng/uL | |
Sunny, Alex | pSB2K3 vector (with K274111 insert) | undigested | 48 uL | 8.8 ng/ul |
color 002 purple insert | digested X,P, gel extracted | 0 ng/uL (no DNA retrieved from gels) | ||
arsR insert | digested E,S | 11.1 ng/uL | ||
S03511 vector (low-medium copy #, inducible) | undigested | 48uL | 41.2 ng/uL | |
I3023 vector (low-medium copy #, inducible) | undigested | 48uL | 97.2 ng/uL | |
I3023 vector (low-medium copy #, inducible) | undigested | 48uL | 93.7 ng/uL | |
I3002 vector (low-medium copy #, constitutive) | undigested | 48uL | 103.9 ng/uL | |
I3002 vector (low-medium copy #, constitutive) | undigested | 48uL | 109.1 ng/uL |
Introduction
TBA
Schedule
TBA