Stanford/BIOE44:Module 4:Day1: Difference between revisions
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What are our options. | What are our options. | ||
Here are some imaginary curves to stimulate your thinking. | Here are some imaginary curves to stimulate your thinking. | ||
[[Image:Imaginary_Curve2.jpg|thumb]] | [[Image:Imaginary_Curve2.jpg|thumb|center]] | ||
[[Image:Imaginary_Curve1.jpg|thumb]] | [[Image:Imaginary_Curve1.jpg|thumb|center]] | ||
===Deconstructing our Device=== | ===Deconstructing our Device=== | ||
[[Image:Deconstruction2.jpg]] | [[Image:Deconstruction2.jpg]] | ||
Revision as of 17:59, 5 May 2010
Moss
Arsenic
Using the Same Language
Please review common symbols for representing your device. http://openwetware.org/wiki/Endy:Notebook/Synthetic_Biology_Open_Language
Current Inventory of Parts
Please enter what you have and indicate the state which it is in.
| Part | status | volume | notes | contributor |
| O/P for ars operon | cPCR, PCR Cleanup, Digested@EcoR:SpeI, Gel Extract | 6ul | ~200ng/ul | Koshlan |
| RBS + arsR ORF | cPCR, PCR Cleanup, Digested@XbaI:PstI, Gel Extracted | 6ul | ~80mg/ul (extra codon flanking ATG) | Koshlan |
| O/P + RBS + arsR (Combined like the edinburgh part) | cPCR, PCR Cleanup, Digested@EcoR:SpeI, PCR Cleanup | 20ul | ~40ng/ul questionable quality since no gel extract | Koshlan |
Design and Testing Goal
We need to think about the fundamental relationship between the amount of pollution in our sample and the reporter strength. What will this relationship look like. How can we alter it? Consider the effect of the following.
- copy number
- ribosome binding site
- ratio of ArsR transcription factor to the operator site
- what else
What are our options.
Here are some imaginary curves to stimulate your thinking.
Deconstructing our Device
