Stanford/M4: Day 3 - Moss Datasheet: Difference between revisions

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*Using Liquid Medium
*Using Liquid Medium


==Cryogenic Freezing of Phycomitrella patens==
==Storing Phycomitrella patens==


[http://cshprotocols.cshlp.org/cgi/content/full/protocols;2009/2/pdb.prot5136 Culturing Moss]
[http://cshprotocols.cshlp.org/cgi/content/full/protocols;2009/2/pdb.prot5136 Culturing Moss]

Revision as of 14:32, 13 May 2010

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M4: Day 3 - Moss Datasheet

Moss Group

Things to think about:

  • uses and advantages of this organism
  • culture methods/procedures
  • status of genetic engineering tools (genetically tractable? transformation procedure? parts available?)
  • important references


Culturing Phycomitrella patens

Culturing Moss

Procedure I: Growth of Gametophytes

  • Using Petri Dishes Containing Solid Medium
  • Using Petri Dishes Containing Solid Medium Overlaid with Cellophane
  • Using Liquid Medium

Storing Phycomitrella patens

Culturing Moss

Procedure II: Long-Term Storage of Gametophyte Tissue

  • Storage on Solid Medium
  • Cryopreservation




Things to think about:

  • uses and advantages of this organism
  • culture methods/procedures
  • status of genetic engineering tools (genetically tractable? transformation procedure? parts available?)
  • important references