Stanford/M4: Day 3 - Moss Datasheet: Difference between revisions
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[[Uses and Advantages of ''Physcomitrella Patens'']] | [[Uses and Advantages of ''Physcomitrella Patens'']] | ||
Physcomitrella patens is a useful organism in research because of its high recombination efficiency. This feature allows researchers to easily introduce and knockout genes into the organism. | Physcomitrella patens is a useful organism in research because of its high recombination efficiency. This feature allows researchers to easily introduce and knockout genes into the organism. Additionally, the facility at which Physcomitrella Patens is grown enables the organism to function as an efficient bioreactor, cheaply producing complex biopharmaceutical therapeutics. | ||
*culture methods/procedures | *culture methods/procedures | ||
Revision as of 15:46, 18 May 2010
M4: Day 3 - Moss Datasheet
Moss-ay Group
Uses and Advantages of ''Physcomitrella Patens''
Physcomitrella patens is a useful organism in research because of its high recombination efficiency. This feature allows researchers to easily introduce and knockout genes into the organism. Additionally, the facility at which Physcomitrella Patens is grown enables the organism to function as an efficient bioreactor, cheaply producing complex biopharmaceutical therapeutics.
- culture methods/procedures
- status of genetic engineering tools (genetically tractable? transformation procedure? parts available?)
- important references
Culturing Phycomitrella patens
Procedure I: Growth of Gametophytes
- Using Petri Dishes Containing Solid Medium
- Using Petri Dishes Containing Solid Medium Overlaid with Cellophane
- Using Liquid Medium
Storing Phycomitrella patens
Procedure II: Long-Term Storage of Gametophyte Tissue
- Storage on Solid Medium
- Cryopreservation
Transforming Phycomitrella patens
Important references
Cove, David J. "The Moss Physcomitrella patens: A Novel Model System for Plant Development and Genomic Studies." Emerging Model Organisms: A Laboratory Manual. 1. (2009): 69-88. Print.
Ralf Reski and Wolfgang Frank (2005) "Moss (Physcomitrella patens) functional genomics – Gene discovery and tool development with implications for crop plants and human health." Briefings in Functional Genomics and Proteomics 4, 48-57.
