Stanford/M4: Day 3 - Moss Datasheet: Difference between revisions
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Important references | Important references | ||
Cove, David J. '''"The Moss Physcomitrella patens: A Novel Model System for Plant Development and Genomic Studies."''' Emerging Model Organisms: A Laboratory Manual. 1. (2009): 69-88. Print. | Cove, David J. '''"The Moss Physcomitrella patens: A Novel Model System for Plant Development and Genomic Studies."''' Emerging Model Organisms: A Laboratory Manual. 1. (2009): 69-88. Print. | ||
Ralf Reski and Wolfgang Frank (2005) '''"Moss (Physcomitrella patens) functional genomics – Gene discovery and tool development with implications for crop plants and human health."''' Briefings in Functional Genomics and Proteomics 4, 48-57. | Ralf Reski and Wolfgang Frank (2005) '''"Moss (Physcomitrella patens) functional genomics – Gene discovery and tool development with implications for crop plants and human health."''' Briefings in Functional Genomics and Proteomics 4, 48-57. | ||
Revision as of 15:44, 18 May 2010
M4: Day 3 - Moss Datasheet
Moss-ay Group
Uses and Advantages of ''Physcomitrella Patens''
Physcomitrella patens is a useful organism in research because of its high recombination efficiency. This feature allows researchers to easily introduce and knockout genes into the organism.
- culture methods/procedures
- status of genetic engineering tools (genetically tractable? transformation procedure? parts available?)
- important references
Culturing Phycomitrella patens
Procedure I: Growth of Gametophytes
- Using Petri Dishes Containing Solid Medium
- Using Petri Dishes Containing Solid Medium Overlaid with Cellophane
- Using Liquid Medium
Storing Phycomitrella patens
Procedure II: Long-Term Storage of Gametophyte Tissue
- Storage on Solid Medium
- Cryopreservation
Transforming Phycomitrella patens
Important references
Cove, David J. "The Moss Physcomitrella patens: A Novel Model System for Plant Development and Genomic Studies." Emerging Model Organisms: A Laboratory Manual. 1. (2009): 69-88. Print.
Ralf Reski and Wolfgang Frank (2005) "Moss (Physcomitrella patens) functional genomics – Gene discovery and tool development with implications for crop plants and human health." Briefings in Functional Genomics and Proteomics 4, 48-57.
