Stephanopoulos:Flux Determination: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
One of the core convictions of our lab is that the study of metabolic fluxes and their relationship to other "omics" data is a prerequisite to understanding cellular physiology. Unlike metabolite, protein and mRNA measurements, which provide information about what is present inside the cell at a particular time, fluxes give insights into what the cell is actually doing with these molecules. They give us a traffic report that describes the flow of material throughout metabolism. This understanding can in turn provide clues as to how to the cells should be manipulated in order to elicit a desired phenotype. | One of the core convictions of our lab is that the study of metabolic fluxes and their relationship to other "omics" data is a prerequisite to understanding cellular physiology. Unlike metabolite, protein and mRNA measurements, which provide information about what is present inside the cell at a particular time, fluxes give insights into what the cell is actually doing with these molecules. They give us a traffic report that describes the flow of material throughout metabolism. This understanding can in turn provide clues as to how to the cells should be manipulated in order to elicit a desired phenotype. | ||
The flux maps we generate rely upon the marriage of precise experimental techniques and sophisticated computational algorithms for subsequent data analysis. Isotopic tracers that incorporate heavy atoms (e.g. <math>^13</math>C or <math>^2</math>H) | The flux maps we generate rely upon the marriage of precise experimental techniques and sophisticated computational algorithms for subsequent data analysis. Isotopic tracers that incorporate heavy atoms (e.g. <math>C^13</math>C or <math>H^2</math>H) | ||
Revision as of 10:46, 14 July 2006
One of the core convictions of our lab is that the study of metabolic fluxes and their relationship to other "omics" data is a prerequisite to understanding cellular physiology. Unlike metabolite, protein and mRNA measurements, which provide information about what is present inside the cell at a particular time, fluxes give insights into what the cell is actually doing with these molecules. They give us a traffic report that describes the flow of material throughout metabolism. This understanding can in turn provide clues as to how to the cells should be manipulated in order to elicit a desired phenotype.
The flux maps we generate rely upon the marriage of precise experimental techniques and sophisticated computational algorithms for subsequent data analysis. Isotopic tracers that incorporate heavy atoms (e.g. [math]\displaystyle{ C^13 }[/math]C or [math]\displaystyle{ H^2 }[/math]H)
