Stephanopoulos:Projects: Difference between revisions

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=<font style="color:maroon">Research</font>=


Our research is focused on Metabolic Engineering - the improvement of cellular properties, using modern genetic tools. This field encompasses two important components:
Our research is focused on Metabolic Engineering - the improvement of cellular properties, using modern genetic tools. This field encompasses two important components:
* the modification of biochemical pathways inside cells and
 
* the rigorous evaluation of the resulting cellular phenotypes.
:* The modification of biochemical pathways inside cells and
<p>
:* The rigorous evaluation of the resulting cellular phenotypes.
 
   
   
Our most recent research has been focused on the following topics:
Our most recent research has been focused on the following topics:


* [[Stephanopoulos Lab:Production of biochemicals in E. coli | '''Metabolic Engineering of <i>E.Coli</i> for the production of biochemicals''']]
:* [[Stephanopoulos Lab:Production of biochemicals in E. coli | '''Metabolic Engineering of <i>E.Coli</i> for the production of biochemicals''']]
 
:* [[Stephanopoulos Lab:Inverse Metabolic Engineering | '''Inverse Metabolic Engineering''']]
 
:* [[Stephanopoulos Lab:gTME | '''gTME''']]
 
:* [[Stephanopoulos Lab:Flux Determination | '''Flux Determination''']]
 
:* [[Stephanopoulos Lab:Hepatocyte Physiology | '''Hepatocyte Physiology''']]
 
:* [[Stephanopoulos Lab:Metabolomics | '''Metabolomics''']]
 
:* [[Stephanopoulos Lab:Systems Biology | '''Systems Biology''']]


* [[Stephanopoulos Lab:Inverse Metabolic Engineering | '''Inverse Metabolic Engineering''']]


* [[Stephanopoulos Lab:gTME | '''gTME''']]


* [[Stephanopoulos Lab:Flux Determination | '''Flux Determination''']]
To accomplish the above goals we make use of a diverse array of scientific tools and
methods, many of which have also become areas of research for our group:


* [[Stephanopoulos Lab:Hepatocyte Physiology | '''Hepatocyte Physiology''']]
:* [[Stephanopoulos Lab:Bioinformatics and Systems Biology | '''Bioinformatics and Systems Biology''']] - Our group was one of the first to realize the importance of computational tools for handling the large volume of data generated by microarrays and other technologies.


* [[Stephanopoulos Lab:Metabolomics | '''Metabolomics''']]
:* [[Stephanopoulos Lab:Methods for intracellular flux determination | '''Methods for intracellular flux determination''']] - Fluxes are determined by material balancing, NMR fine spectra analysis and GC-MS measurements.


* [[Stephanopoulos Lab:Systems Biology | '''Systems Biology''']]
:* [[Stephanopoulos Lab:DNA microarrays | '''DNA microarrays''']] - We have developed full genome microarrays for ''Synechocystis'' Sp., and partial microarrays for ''C. glutamicum'', ''E. coli'', and the mouse genomes.


<html>
:* [[Stephanopoulos Lab:Bioreaction network analysis | '''Bioreaction network analysis''']]
                <br>
            To accomplish the above goals we make use of a diverse array of scientific tools and
methods, many of which have also become areas of research for our group:
                <ul>
                <p>
                <li>
                <b><a href="Bioinfo.shtml">Bioinformatics and Systems Biology</a></b> - Our group was
one of the first to realize the importance of computational tools for handling the
large volume of data generated by microarrays and other technologies.
                <li>
<b><a href="Fluxes.shtml">Methods for intracellular flux determination</a></b>
                - Fluxes are determined by material balancing, NMR fine spectra analysis and
GC-MS measurements.
                <li>
                <b><a href="Microarray.shtml">DNA microarrays</a></b> - We have developed full
genome microarrays for <i>Synechocystis</i> Sp., and partial microarrays for
                <i>C. glutamicum</i>, <i>E. coli</i>, and the mouse genomes.
                <li>
                <b><a href="BioReactor.shtml">Bioreaction network analysis.</a></b>
<br>


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      <font color="maroon"><tt>Address</tt></font><br>
      <small>
Massachusetts Institute of Technology<br>
Department of Chemical Engineering<br>
Cambridge, MA 02139<br>
Room 56-469
      </small>
  </td>
  <td align="center" width="250" valign="top">
      <small>
<a href="http://www.mit.edu">MIT</a> | <a href="/wiki/Stephanopoulos_Lab">HOME</a><br>
Last Modified:<br>Monday, July 10th, 2006 - by <a href="Adrian.shtml">afi</a>.
      </small>
      <br>
      <font color="#990000"><tt>gnswebpage-www@mit.edu</tt></font>
  </td>
  <td align="right" width="250" valign="top">
      <font color="#990000"><tt>Telephones</tt><br></font>
      <small>
office | 617.258.0398<br>
lab | 617.253.6591<br>
lab | 617.258.0349<br>
lab | 617.253.6521<br>
fax | 617.253.3122<br>
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Revision as of 15:49, 21 May 2007


Stephanopoulos Lab logo

Research

Our research is focused on Metabolic Engineering - the improvement of cellular properties, using modern genetic tools. This field encompasses two important components:

  • The modification of biochemical pathways inside cells and
  • The rigorous evaluation of the resulting cellular phenotypes.


Our most recent research has been focused on the following topics:


To accomplish the above goals we make use of a diverse array of scientific tools and methods, many of which have also become areas of research for our group:

  • Bioinformatics and Systems Biology - Our group was one of the first to realize the importance of computational tools for handling the large volume of data generated by microarrays and other technologies.
  • DNA microarrays - We have developed full genome microarrays for Synechocystis Sp., and partial microarrays for C. glutamicum, E. coli, and the mouse genomes.

Address

Department of Chemical Engineering

Massachusetts Institute of Technology

77 Massachusetts Ave,

Room 56-469

Cambridge, MA 02139

MIT | HOME

Last Modified:
Monday, March 3, 2008 - by afi.

gnswebpage-www@mit.edu

Telephones

office | 617.258.0398

lab | 617.253.6591

lab | 617.258.0349

lab | 617.253.6521

fax | 617.253.3122

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