Stephanopoulos:Projects

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=<font style="color:maroon">Research</font>=
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= <font style="color:maroon">Research</font> =
Our research is focused on Metabolic Engineering - the improvement of cellular properties, using modern genetic tools. This field encompasses two important components:
Our research is focused on Metabolic Engineering - the improvement of cellular properties, using modern genetic tools. This field encompasses two important components:
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:* '''The modification of biochemical pathways inside cells''' and
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# '''The modification of biochemical pathways inside cells''', and
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:* '''The rigorous evaluation of the resulting cellular phenotypes.'''
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# '''The rigorous evaluation of the resulting cellular phenotypes.'''
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Our most recent research has been focused on the following topics:
Our most recent research has been focused on the following topics:
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:* [[Stephanopoulos Lab:Production of biochemicals in E. coli | '''Metabolic Engineering of <i>E.Coli</i> for the production of biochemicals''']]
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:* [[Stephanopoulos:Production of biochemicals in E. coli | '''Metabolic Engineering of <i>E.Coli</i> for the production of biochemicals''']]
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:* [[Stephanopoulos Lab:Inverse Metabolic Engineering | '''Inverse Metabolic Engineering''']]
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:* [[Stephanopoulos:Inverse Metabolic Engineering | '''Inverse Metabolic Engineering''']]
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:* [[Stephanopoulos Lab:gTME | '''gTME''']]
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:* [[Stephanopoulos:gTME | '''gTME''']]
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:* [[Stephanopoulos Lab:Flux Determination | '''Flux Determination''']]
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:* [[Stephanopoulos:Flux Determination | '''Flux Determination''']]
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:* [[Stephanopoulos Lab:Hepatocyte Physiology | '''Hepatocyte Physiology''']]
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:* [[Stephanopoulos:Hepatocyte Physiology | '''Hepatocyte Physiology''']]
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:* [[Stephanopoulos Lab:Metabolomics | '''Metabolomics''']]
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:* [[Stephanopoulos:Metabolomics | '''Metabolomics''']]
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:* [[Stephanopoulos Lab:Rational Drug Design | '''Rational Drug Design''']]
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:* [[Stephanopoulos:Rational Drug Design | '''Rational Drug Design''']]
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:* [[Stephanopoulos Lab:Systems Biology | '''Systems Biology''']]
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:* [[Stephanopoulos:Systems Biology | '''Systems Biology''']]
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methods, many of which have also become areas of research for our group:
methods, many of which have also become areas of research for our group:
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:* [[Stephanopoulos Lab:Bioinformatics and Systems Biology | '''Bioinformatics and Systems Biology''']] - Our group was one of the first to realize the importance of computational tools for handling the large volume of data generated by microarrays and other technologies.
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:* [[Stephanopoulos:Bioinformatics and Systems Biology | '''Bioinformatics and Systems Biology''']] - Our group was one of the first to realize the importance of computational tools for handling the large volume of data generated by microarrays and other technologies.
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:* [[Stephanopoulos Lab:Methods for intracellular flux determination | '''Methods for intracellular flux determination''']] - Fluxes are determined by material balancing, NMR fine spectra analysis and GC-MS measurements.
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:* [[Stephanopoulos:Methods for intracellular flux determination | '''Methods for intracellular flux determination''']] - Fluxes are determined by material balancing, NMR fine spectra analysis and GC-MS measurements.
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:* [[Stephanopoulos Lab:DNA microarrays | '''DNA microarrays''']] - We have developed full genome microarrays for ''Synechocystis'' Sp., and partial microarrays for ''C. glutamicum'', ''E. coli'', and the mouse genomes.  
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:* [[Stephanopoulos:DNA microarrays | '''DNA microarrays''']] - We have developed full genome microarrays for ''Synechocystis'' Sp., and partial microarrays for ''C. glutamicum'', ''E. coli'', and the mouse genomes.  
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:* [[Stephanopoulos Lab:Bioreaction network analysis | '''Bioreaction network analysis''']]
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:* [[Stephanopoulos:Bioreaction network analysis | '''Bioreaction network analysis''']]
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Research

Our research is focused on Metabolic Engineering - the improvement of cellular properties, using modern genetic tools. This field encompasses two important components:

  1. The modification of biochemical pathways inside cells, and
  2. The rigorous evaluation of the resulting cellular phenotypes.


Our most recent research has been focused on the following topics:


To accomplish the above goals we make use of a diverse array of scientific tools and methods, many of which have also become areas of research for our group:

  • Bioinformatics and Systems Biology - Our group was one of the first to realize the importance of computational tools for handling the large volume of data generated by microarrays and other technologies.
  • DNA microarrays - We have developed full genome microarrays for Synechocystis Sp., and partial microarrays for C. glutamicum, E. coli, and the mouse genomes.

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