Stephanopoulos:Projects

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<b>b)</b> the rigorous evaluation of the resulting cellular phenotypes.
<b>b)</b> the rigorous evaluation of the resulting cellular phenotypes.
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=== Our most recent research has been focused on the following topics: ===
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* Metabolic Engineering of <i>E.Coli</i> for the production of biochemicals
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* Inverse Metabolic Engineering
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Our most recent research has been focussed on the following topics:
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                <ul>
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<li><b><a href="Biochemicals.shtml">Metabolic Engineering of <i>E.Coli</i>
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                for the production of biochemicals.</b></a>
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<li><b><a href="Inverse.shtml">Inverse Metabolic Engineering</b></a>
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* gTME
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<li><b><a href="gTME.shtml">gTME</b></a>
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* Flux Determination
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                <li><b><a href="Flux.shtml">Flux Determination</b></a>
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<li><b><a href="Hepatocyte.shtml">Hepatocyte Physiology</b></a>
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* Hepatocyte Physiology
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* Metabolomics
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* Systems Biology
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<li><b><a href="Metabolomics.shtml">Metabolomics</b></a>
 
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<li><b><a href="SysBio.shtml">Systems Biology</b></a>
 
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                </ul>
 
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             To accomplish the above goals we make use of a diverse array of scientific tools and
             To accomplish the above goals we make use of a diverse array of scientific tools and

Revision as of 17:42, 12 July 2006

Research

Our research is focused on Metabolic Engineering - the improvement of cellular properties, using modern genetic tools. This field encompasses two important components: a) the modification of biochemical pathways inside cells and b) the rigorous evaluation of the resulting cellular phenotypes.

Our most recent research has been focused on the following topics:

  • Metabolic Engineering of E.Coli for the production of biochemicals
  • Inverse Metabolic Engineering
  • gTME
  • Flux Determination
  • Hepatocyte Physiology
  • Metabolomics
  • Systems Biology


               
To accomplish the above goals we make use of a diverse array of scientific tools and

methods, many of which have also become areas of research for our group:

  • <a href="Bioinfo.shtml">Bioinformatics and Systems Biology</a> - Our group was one of the first to realize the importance of computational tools for handling the large volume of data generated by microarrays and other technologies.
  • <a href="Fluxes.shtml">Methods for intracellular flux determination</a> - Fluxes are determined by material balancing, NMR fine spectra analysis and GC-MS measurements.
  • <a href="Microarray.shtml">DNA microarrays</a> - We have developed full genome microarrays for Synechocystis Sp., and partial microarrays for C. glutamicum, E. coli, and the mouse genomes.
  • <a href="BioReactor.shtml">Bioreaction network analysis.</a>
    </html>


Address
Massachusetts Institute of Technology
Department of Chemical Engineering
Cambridge, MA 02139
Room 56-469
MIT | HOME
Last Modified:
Monday, July 10th, 2006 - by afi.

gnswebpage-www@mit.edu
Telephones
office | 617.258.0398
lab | 617.253.6591
lab | 617.258.0349
lab | 617.253.6521
fax | 617.253.3122
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