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Research

Our research is focused on Metabolic Engineering - the improvement of cellular properties, using modern genetic tools. This field encompasses two important components: a) the modification of biochemical pathways inside cells and b) the rigorous evaluation of the resulting cellular phenotypes. In recent years our group has investigated the following topics:

• Aminoacid biosynthesis in Corynebacterium glutamicum.
• Indene bioconversion in Rhodococcus Sp.
• CO₂ fixation and product synthesis by the cyanobacterium Synechocystis Sp.
• Elucidating type II diabetes by linking the expression and metabolic phenotypes of hepatoma and hepatocyte cells.

To accomplish the above goals we make use of a diverse array of scientific tools and methods, many of which have also become areas of research for our group:

• Bioinformatics and Systems Biology - Our group was one of the first to realize the importance of computational tools for handling the large volume of data generated by microarrays and other technologies.
• Methods for intracellular flux determination - Fluxes are determined by material balancing, NMR fine spectra analysis and GC-MS measurements.
• DNA microarrays - We have developed full genome microarrays for Synechocystis Sp., and partial microarrays for C. glutamicum, E. coli, and the mouse genomes.
• Bioreaction network analysis.
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