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(New page: ==Overview== Counting cells with a hemocytometer. ==Materials== *Tube of cell suspension *Hemocytometer *Trypan Blue 0.4% ==Procedure== #Combine 20 ul cell suspension with 20ul of Tryp...)
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Revision as of 13:38, 28 August 2007
Counting cells with a hemocytometer.
- Tube of cell suspension
- Trypan Blue 0.4%
- Combine 20 ul cell suspension with 20ul of Trypan Blue. Mix.
- Load 10ul into each of the 2 hemocytometer "sides" using the capillary action created by the coverslip and the hemocytometer. Avoid bubbles.
- Count the number of cells in the gridded area of each side. (See below) White cells are live and blue cells are dead/dying.
- Record cell count and repeat for the other side of the hemocytometer.
- If the two cell counts are roughly equivalent, average the cell counts. Otherwise, recount.
- Mean cell count/4 * Dilution = Cells x 104/ml.
- Above example, Mean cell count/4 * 2 = Cells x 104/ml.