Streptomyces:Media and Solutions: Difference between revisions

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|- style="text-align:center;" valign="middle"|
|- style="text-align:center;" valign="middle"|
| Ampicillin || 100 || 100 || sdH<sub>2</sub>0
| Ampicillin || 100 || 100 || sdH<sub>2</sub>0
|-
|- style="text-align:center;" valign="middle"|
| Apramycin || 100 || 50 || sdH<sub>2</sub>0
| Apramycin || 100 || 50 || sdH<sub>2</sub>0
|-
|- style="text-align:center;" valign="middle"|
| Chloramphenicol || 25 || 25 || 50%-100% EtOH
| Chloramphenicol || 25 || 25 || 50%-100% EtOH
|-
|- style="text-align:center;" valign="middle"|
| Hygromycin || 50 || 25 || sdH<sub>2</sub>0
| Hygromycin || 50 || 25 || sdH<sub>2</sub>0
|-
|- style="text-align:center;" valign="middle"|
| Kanamycin || 100 || 50 || sdH<sub>2</sub>0
| Kanamycin || 100 || 50 || sdH<sub>2</sub>0
|-
|- style="text-align:center;" valign="middle"|
| Nalidixic Acid || 25 || 25 || 0.2M NaOH
| Nalidixic Acid || 25 || 25 || 0.2M NaOH
|-
|- style="text-align:center;" valign="middle"|
| Thiostrepton(e) || 50 || 50 || 100% DMSO or 1:1 DMSO:EtOH
| Thiostrepton(e) || 50 || 50 || 100% DMSO or 1:1 DMSO:EtOH
|-
|- style="text-align:center;" valign="middle"|
| Viomycin || 30 || 30 || sdH<sub>2</sub>0
| Viomycin || 30 || 30 || sdH<sub>2</sub>0
|}
|}

Revision as of 04:07, 25 May 2007

Media and Solutions

Streptomyces @ UEA

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Media and Solutions

Antibiotic Concentrations and Usage

Name Stock Concentration (mg/ml) Final Concentration (μg/ml) Dissolve in
Ampicillin 100 100 sdH20
Apramycin 100 50 sdH20
Chloramphenicol 25 25 50%-100% EtOH
Hygromycin 50 25 sdH20
Kanamycin 100 50 sdH20
Nalidixic Acid 25 25 0.2M NaOH
Thiostrepton(e) 50 50 100% DMSO or 1:1 DMSO:EtOH
Viomycin 30 30 sdH20