Streptomyces:Media and Solutions: Difference between revisions

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{|cellspacing="5" cellpadding="0" border="0" style="width: 770px;"
{|cellspacing="5" cellpadding="0" border="0" style="width: 770px;"
|- style="text-align:center;" valign="top"|
|- style="text-align:center;" valign="top"
! style="width:175px;" | Name || style="width:150px;" | Stock Concentration (mg/ml) || style="width:150px;"| Final Concentration (μg/ml) || style="width:100px;"| Usage in 10mL (μL) || Dissolve in
! style="width:175px;" | Name || style="width:150px;" | Stock Concentration (mg/ml) || style="width:150px;"| Final Concentration (μg/ml) || style="width:100px;"| Usage in 10mL (μL) || Dissolve in
|- style="text-align:center;" valign="middle"|
|- style="text-align:center;"
| Ampicillin || 100 || 100 || 10 || sdH<sub>2</sub>0
| Ampicillin || 100 || 100 || 10 || sdH<sub>2</sub>0
|- style="text-align:center;" valign="middle"|
|- style="text-align:center;"
| Apramycin || 100 || 50 || 5 || sdH<sub>2</sub>0
| Apramycin || 100 || 50 || 5 || sdH<sub>2</sub>0
|- style="text-align:center;" valign="middle"|
|- style="text-align:center;"
| Chloramphenicol || 25 || 25 || 10 || 50%-100% EtOH
| Chloramphenicol || 25 || 25 || 10 || 50%-100% EtOH
|- style="text-align:center;" valign="middle"|
|- style="text-align:center;"
| Hygromycin || 50 || 25 || 5 || sdH<sub>2</sub>0
| Hygromycin || 50 || 25 || 5 || sdH<sub>2</sub>0
|- style="text-align:center;" valign="middle"|
|- style="text-align:center;"
| Kanamycin || 100 || 50 || 5 || sdH<sub>2</sub>0
| Kanamycin || 100 || 50 || 5 || sdH<sub>2</sub>0
|- style="text-align:center;" valign="middle"|
|- style="text-align:center;"
| Nalidixic Acid || 25 || 25 || 10 || 0.2M NaOH
| Nalidixic Acid || 25 || 25 || 10 || 0.2M NaOH
|- style="text-align:center;" valign="middle"|
|- style="text-align:center;"
| Thiostrepton(e) || 50 || 50 || 10 || 100% DMSO or 1:1 DMSO:EtOH
| Thiostrepton(e) || 50 || 50 || 10 || 100% DMSO or 1:1 DMSO:EtOH
|- style="text-align:center;" valign="middle"|
|- style="text-align:center;"
| Viomycin || 30 || 30 || 10 || sdH<sub>2</sub>0
| Viomycin || 30 || 30 || 10 || sdH<sub>2</sub>0
|}
|}

Revision as of 02:54, 12 June 2007

Media and Solutions

Streptomyces @ UEA

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Media and Solutions

Use the links below to view / download the protocol.

File Type Download / Description


Antibiotic Concentrations and Usage

Name Stock Concentration (mg/ml) Final Concentration (μg/ml) Usage in 10mL (μL) Dissolve in
Ampicillin 100 100 10 sdH20
Apramycin 100 50 5 sdH20
Chloramphenicol 25 25 10 50%-100% EtOH
Hygromycin 50 25 5 sdH20
Kanamycin 100 50 5 sdH20
Nalidixic Acid 25 25 10 0.2M NaOH
Thiostrepton(e) 50 50 10 100% DMSO or 1:1 DMSO:EtOH
Viomycin 30 30 10 sdH20