Streptomyces:Media and Solutions: Difference between revisions

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{{Streptomyces}}
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== Media and Solutions ==
== Media and Solutions ==


===Antibiotic Concentrations and Usage===
'''Soya Flour Mannitol Agar''' (Also known as MS or SFM)


{|cellspacing="5" cellpadding="0" border="0" style="width: 770px;"
Add 2 grams of soya flour and 2 grams of agar to 10 x 250 ml conical flasks. Dissolve 20 grams mannitol in 1 litre of tap water. Dispense 100 ml into each of the 10 flasks, add bungs and foil and autoclave twice.
|- style="text-align:center;" valign="middle"|
! style="width:200px;" | Name || style="width:150px;" | Stock Concentration (mg/ml) || style="width:150px;"| Final Concentration (&#956;g/ml) || Dissolve in
|- style="text-align:center;" valign="middle"|
| Ampicillin || 100 || 100 || sdH<sub>2</sub>0
|- style="text-align:center;" valign="middle"|
| Apramycin || 100 || 50 || sdH<sub>2</sub>0
|- style="text-align:center;" valign="middle"|
| Chloramphenicol || 25 || 25 || 50%-100% EtOH
|- style="text-align:center;" valign="middle"|
| Hygromycin || 50 || 25 || sdH<sub>2</sub>0
|- style="text-align:center;" valign="middle"|
| Kanamycin || 100 || 50 || sdH<sub>2</sub>0
|- style="text-align:center;" valign="middle"|
| Nalidixic Acid || 25 || 25 || 0.2M NaOH
|- style="text-align:center;" valign="middle"|
| Thiostrepton(e) || 50 || 50 || 100% DMSO or 1:1 DMSO:EtOH
|- style="text-align:center;" valign="middle"|
| Viomycin || 30 || 30 || sdH<sub>2</sub>0
|}


'''Oatmeal agar''' (for ''S. scabies'')


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Add 4 grams of rolled oats and 2 grams of agar to 10 x 250 ml conical flasks. Add 100 ml tap water to each of the 10 flasks, add bungs and foil and autoclave twice.
|}<br style="clear:both" />
 
'''Instant Potatomash Medium'''  (for ''S. scabies'')
 
Add 2 grams of agar to 10 x 250 ml conical flasks. Boil 1 litre of tap water and dissolve in 50 grams of Smash powder.  Dispense 100 ml into each of the 10 flasks, add bungs and foil and autoclave twice.

Latest revision as of 08:09, 11 October 2007

Media and Solutions

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Media and Solutions

Soya Flour Mannitol Agar (Also known as MS or SFM)

Add 2 grams of soya flour and 2 grams of agar to 10 x 250 ml conical flasks. Dissolve 20 grams mannitol in 1 litre of tap water. Dispense 100 ml into each of the 10 flasks, add bungs and foil and autoclave twice.

Oatmeal agar (for S. scabies)

Add 4 grams of rolled oats and 2 grams of agar to 10 x 250 ml conical flasks. Add 100 ml tap water to each of the 10 flasks, add bungs and foil and autoclave twice.

Instant Potatomash Medium (for S. scabies)

Add 2 grams of agar to 10 x 250 ml conical flasks. Boil 1 litre of tap water and dissolve in 50 grams of Smash powder. Dispense 100 ml into each of the 10 flasks, add bungs and foil and autoclave twice.

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