Streptomyces:Media and Solutions: Difference between revisions
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== Media and Solutions == | == Media and Solutions == | ||
'''Soya Flour Mannitol Agar''' (Also known as MS or SFM) | |||
Add 2 grams of soya flour and 2 grams of agar to 10 x 250 ml conical flasks. Dissolve 20 grams mannitol in 1 litre of tap water. Dispense 100 ml into each of the 10 flasks, add bungs and foil and autoclave twice. | |||
'''Oatmeal agar''' (for ''S. scabies'') | |||
Add 4 grams of rolled oats and 2 grams of agar to 10 x 250 ml conical flasks. Add 100 ml tap water to each of the 10 flasks, add bungs and foil and autoclave twice. | |||
'''Instant Potatomash Medium''' (for ''S. scabies'') | |||
Add 2 grams of agar to 10 x 250 ml conical flasks. Boil 1 litre of tap water and dissolve in 50 grams of Smash powder. Dispense 100 ml into each of the 10 flasks, add bungs and foil and autoclave twice. |
Latest revision as of 08:09, 11 October 2007
Media and Solutions
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Media and SolutionsSoya Flour Mannitol Agar (Also known as MS or SFM) Add 2 grams of soya flour and 2 grams of agar to 10 x 250 ml conical flasks. Dissolve 20 grams mannitol in 1 litre of tap water. Dispense 100 ml into each of the 10 flasks, add bungs and foil and autoclave twice. Oatmeal agar (for S. scabies) Add 4 grams of rolled oats and 2 grams of agar to 10 x 250 ml conical flasks. Add 100 ml tap water to each of the 10 flasks, add bungs and foil and autoclave twice. Instant Potatomash Medium (for S. scabies) Add 2 grams of agar to 10 x 250 ml conical flasks. Boil 1 litre of tap water and dissolve in 50 grams of Smash powder. Dispense 100 ml into each of the 10 flasks, add bungs and foil and autoclave twice. |