Streptomyces:Media and Solutions: Difference between revisions

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{|cellspacing="10" cellpadding="0" border="1" style="width: 770px;"
{|cellspacing="10" cellpadding="0" border="1" style="width: 770px;"
|- style="text-align:center;" valign="middle"|
! style="width:200px;" | Name || style="width:150px;" | Stock Concentration (mg/ml) || style="width:150px;"| Final Concentration (μg/ml) || Dissolve in
|- style="text-align:center;" valign="middle"|
| Ampicillin || 100 || 100 || sdH<sub>2</sub>0
|-
|-
! style="width: 40px; text-align: center; valign: bottom;"| Name || style="text-align: left;" valign="bottom"| Stock Concentration (mg/ml)|| style="text-align: left;" valign="bottom"| Final (usage) Concentration (&#956;g/ml)
| Apramycin || 100 || 50 || sdH<sub>2</sub>0
|-
|-
|
| Chloramphenicol || 25 || 25 || 50%-100% EtOH
|-
|-
|
| Hygromycin || 50 || 25 || sdH<sub>2</sub>0
|-
| Kanamycin || 100 || 50 || sdH<sub>2</sub>0
|-
| Nalidixic Acid || 25 || 25 || 0.2M NaOH
|-
| Thiostrepton(e) || 50 || 50 || 100% DMSO or 1:1 DMSO:EtOH
|-
| Viomycin || 30 || 30 || sdH<sub>2</sub>0
|}
|}



Revision as of 04:06, 25 May 2007

Media and Solutions

Streptomyces @ UEA

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Media and Solutions

Antibiotic Concentrations and Usage

Name Stock Concentration (mg/ml) Final Concentration (μg/ml) Dissolve in
Ampicillin 100 100 sdH20
Apramycin 100 50 sdH20
Chloramphenicol 25 25 50%-100% EtOH
Hygromycin 50 25 sdH20
Kanamycin 100 50 sdH20
Nalidixic Acid 25 25 0.2M NaOH
Thiostrepton(e) 50 50 100% DMSO or 1:1 DMSO:EtOH
Viomycin 30 30 sdH20