Streptomyces:Other Bits/Antibiotic and Resistance Marker Information: Difference between revisions
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===Antibiotic Concentrations and Usage=== | ===Antibiotic Concentrations and Usage=== | ||
{|cellspacing=" | {|cellspacing="0" cellpadding="5" rules="rows" frame="hsides" style="width: 770px;" | ||
|- style="text-align:center;" valign="top" | |- style="text-align:center;" valign="top" | ||
! style="width:175px;" | Name || style="width:150px;" | Stock Concentration (mg/ml) || style="width:150px;"| Final Concentration (μg/ml) || style="width:100px;"| Usage in 10mL (μL) || Dissolve in | ! style="width:175px;" | Name || style="width:150px;" | Stock Concentration (mg/ml) || style="width:150px;"| Final Concentration (μg/ml) || style="width:100px;"| Usage in 10mL (μL) || Dissolve in | ||
|- style="text-align:center; | |- style="text-align:center;" | ||
| Ampicillin || 100 || 100 || 10 || sdH<sub>2</sub>0 | | Ampicillin || 100 || 100 || 10 || sdH<sub>2</sub>0 | ||
|- style="text-align:center;" | |- style="text-align:center;" | ||
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===E.coli Strain & Plasmid Resistance Markers=== | ===E.coli Strain & Plasmid Resistance Markers=== | ||
{|cellspacing=" | {|cellspacing="0" cellpadding="5" style="width: 770px;" rules="rows" frame="hsides" | ||
|- style="text-align:center;" valign="top" | |- style="text-align:center;" valign="top" | ||
! style="width:130px;" | E.coli Strain || style="width:120px;" | Plasmid / Cosmid || style="width:120px;"| E.coli Resistance Marker || style="width:100px;"| Plasmid Resistance Marker || Temperature Sensitive Replicon (ts) | ! style="width:130px;" | E.coli Strain || style="width:120px;" | Plasmid / Cosmid || style="width:120px;"| E.coli Resistance Marker || style="width:100px;"| Plasmid Resistance Marker || Temperature Sensitive Replicon (ts) | ||
|- style="text-align:center | |- style="text-align:center;" | ||
| BW25113 || pIJ790 (λRED) || None || ''cat'' || Replication @ 30°C, <br/>Loss of λRED @ 37°C | | BW25113 || pIJ790 (λRED) || None || ''cat'' || Replication @ 30°C, <br/>Loss of λRED @ 37°C | ||
|- style="text-align:center;" | |- style="text-align:center;" | ||
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===Antibiotic Genes & Information=== | ===Antibiotic Genes & Information=== | ||
{|cellspacing=" | {|cellspacing="0" cellpadding="5" style="width: 770px;" rules="rows" frame="hsides" | ||
|- style="text-align:center;" valign="top" | |- style="text-align:center;" valign="top" | ||
! style="width:225px;" | Antibiotic Name || style="width:125px;" | Gene Name || style="width:125px;"| Type || Common Form | ! style="width:225px;" | Antibiotic Name || style="width:125px;" | Gene Name || style="width:125px;"| Type || Common Form | ||
|- style="text-align:center | |- style="text-align:center;" | ||
| Ampicillin || ''bla'' || Bactericidal || Sodium | | Ampicillin || ''bla'' || Bactericidal || Sodium | ||
|- style="text-align:center;" | |- style="text-align:center;" |
Revision as of 05:37, 27 July 2007
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Antibiotic Concentrations and Usage
E.coli Strain & Plasmid Resistance Markers
Antibiotic Genes & Information
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