Streptomyces:Other Bits/Antibiotic and Resistance Marker Information

From OpenWetWare

< Streptomyces:Other Bits(Difference between revisions)
Jump to: navigation, search
Current revision (10:40, 31 August 2007) (view source)
 
(12 intermediate revisions not shown.)
Line 1: Line 1:
-
==Media and Solutions - Antibiotic & Resistance Marker Information==
+
==Other Bits - Antibiotic & Resistance Marker Information==
<!--INSERT THE TEMPLATE HEADER-->
<!--INSERT THE TEMPLATE HEADER-->
{{Streptomyces}}
{{Streptomyces}}
-
<br>
 
{|cellspacing="5" cellpadding="10" style="background:#000; width: 800px;"
{|cellspacing="5" cellpadding="10" style="background:#000; width: 800px;"
|-valign="top"
|-valign="top"
|style="background:#ffffff"|
|style="background:#ffffff"|
 +
<!--THESE CATEGORY LINES ADD THE PROTOCOL TO THE OWW PROTOCOLS PAGE-->
 +
[[Category:Material]]
 +
[[Category:Escherichia coli]]
 +
[[Category:Streptomyces]]
 +
[[Category:Antibiotic]]
<!--CONTENT START-->
<!--CONTENT START-->
-
===Antibiotic Concentrations and Usage===
+
<big>'''Antibiotic Concentrations and Usage'''</big>
-
{|cellspacing="5" cellpadding="0" style="width: 770px; border-collapse:collapse; border-top:hidden; border-top: 2px solid black" rules="rows" frame="hsides"
+
{|cellspacing="0" cellpadding="5" rules="rows" frame="hsides" style="width: 770px;"
-
|- style="text-align:center; border-top: 1px solid black" valign="top"
+
|- style="text-align:center;" valign="top"
! style="width:175px;" | Name || style="width:150px;" | Stock Concentration (mg/ml) || style="width:150px;"| Final Concentration (&#956;g/ml) || style="width:100px;"| Usage in 10mL (&#956;L) || Dissolve in
! style="width:175px;" | Name || style="width:150px;" | Stock Concentration (mg/ml) || style="width:150px;"| Final Concentration (&#956;g/ml) || style="width:100px;"| Usage in 10mL (&#956;L) || Dissolve in
-
|- style="text-align:center;"
+
|- style="text-align:center; border-top: 2px solid black;"
| Ampicillin || 100 || 100 || 10 || sdH<sub>2</sub>0
| Ampicillin || 100 || 100 || 10 || sdH<sub>2</sub>0
|- style="text-align:center;"
|- style="text-align:center;"
Line 30: Line 34:
| Viomycin || 30 || 30 || 10 || sdH<sub>2</sub>0
| Viomycin || 30 || 30 || 10 || sdH<sub>2</sub>0
|}
|}
 +
<br/>
 +
<br/>
 +
<br/>
 +
<big>'''E.coli Strain & Plasmid Resistance Markers'''</big>
-
===E.coli Strain & Plasmid Resistance Markers===
+
{|cellspacing="0" cellpadding="5" style="width: 770px;" rules="rows" frame="hsides"
 +
|- style="text-align:center;" valign="top"
 +
! style="width:130px;" | E.coli Strain || style="width:120px;" | Plasmid / Cosmid || style="width:120px;"| E.coli Resistance Marker || style="width:100px;"| Plasmid Resistance Marker || Temperature Sensitive Replicon <br/>(t<sup>s</sup>)
 +
|- style="text-align:center; border-top: 2px solid black;"
 +
| BW25113 || pIJ790 (λRED) || None || ''cat'' || Replication @ 30&#176;C, <br/>Loss of λRED @ 37&#176;C
 +
|- style="text-align:center;"
 +
| DH5α || None || N/A || ||
 +
|- style="text-align:center;"
 +
| DH5α || BT340 (FLP Recombinase) || None || ''cat'' / ''bla<sub>carb</sub>'' || Initial Replication @ 30&#176;C, <br/>Induction of FLP Recombinase & loss of plasmid @ 42&#176;C, <br/>Post recombination growth @37&#176;C
 +
|- style="text-align:center;"
 +
| ET12567 <br/>[dam-13::Tn9] || pUZ8002 || ''cat'' || ''neo'' ||
 +
|- style="text-align:center;"
 +
| || SuperCos 1 || || ''neo'' / ''bla<sub>carb</sub>'' ||
 +
|- style="text-align:center;"
 +
| || pIJ773 || || ''acc(3)IV'' ||
 +
|}
 +
<br/>
 +
<br/>
 +
<br/>
 +
 
 +
<big>'''Antibiotic Genes & Information'''</big>
-
{|cellspacing="5" cellpadding="0" style="width: 770px; border-collapse:collapse; border-top:hidden;" rules="rows" frame="hsides"
+
{|cellspacing="0" cellpadding="5" style="width: 770px;" rules="rows" frame="hsides"
-
|- style="text-align:center; border-top:1px solid black;" valign="top"  
+
|- style="text-align:center;" valign="top"  
-
! style="width:175px;" | E.coli Strain || style="width:150px;" | Plasmid / Cosmid || style="width:150px;"| E.coli Resistance Marker || style="width:100px;"| Plasmid Resistance Marker || Temperature Sensitive Replicon (ts)
+
! style="width:225px;" | Antibiotic Name || style="width:125px;" | Gene Name || style="width:125px;"| Type || Common Form
 +
|- style="text-align:center; border-top: 2px solid black;"
 +
| Ampicillin || ''bla'' || Bactericidal || Sodium
|- style="text-align:center;"
|- style="text-align:center;"
-
| BW25113 || pIJ790 (λRED) || None || cat || Replication @ 30C, Loss of λRED @ 37C
+
| Apramycin || ''aac(3)IV'' || Aminoglycoside || Sulphate
|- style="text-align:center;"
|- style="text-align:center;"
-
| DH5α || None || N/A || ||
+
| Carbenicillin || ''bla<sub>carb</sub>'' || Bactericidal || DiSodium
 +
|- style="text-align:center;"
 +
| Chloramphenicol || ''cat'' || Bacteriostatic ||
 +
|- style="text-align:center;"
 +
| Erythromycin || ''ermE'' || Macrolides ||
 +
|- style="text-align:center;"
 +
| Hygromycin B || ''hph'' || Aminoglycoside ||
 +
|- style="text-align:center;"
 +
| Kanamycin / Neomycin || ''neo'' || Aminoglycoside || MonoSulphate
 +
|- style="text-align:center;"
 +
| Nalidixic Acid || Synthetic || Bacteriostatic ||
 +
|- style="text-align:center;"
 +
| Spectinomycin / Streptomycin || ''aadA'' || Aminocyclitol || DiHydrochloride Pentahydrate
|- style="text-align:center;"
|- style="text-align:center;"
-
| DH5α || BT340 (FLP Recombinase) || None || cat / blacarb || Initial Replication @ 30C, Induction of FLP Recombinase & loss of plasmid @ 42C, Post recombination growth @37C
+
| Tetracyclin || ''Tet'' || Aminoglycoside || Hydrochloride
|- style="text-align:center;"
|- style="text-align:center;"
-
| ET12567 [dam-13::Tn9] || pUZ8002 || cat || neo ||
+
| Thiostrepton || ''tsr'' || Aminoglycoside ||
|- style="text-align:center;"
|- style="text-align:center;"
-
| || SuperCos 1 || || neo / blacarb ||
+
| Viomycin || ''vph'' || Aminoglycoside? || Sulphate
|- style="text-align:center;"
|- style="text-align:center;"
-
| || pIJ773 || || acc(3)IV ||
+
| Vancomycin B || ''vanB'' || Glycopeptide || Hydrochloride
|}
|}

Current revision

Other Bits - Antibiotic & Resistance Marker Information

Streptomyces @ UEA

Home        Research        Research Groups        Publications        Resources        Meetings        Links        Contact

Antibiotic Concentrations and Usage

Name Stock Concentration (mg/ml) Final Concentration (μg/ml) Usage in 10mL (μL) Dissolve in
Ampicillin 100 100 10 sdH20
Apramycin 100 50 5 sdH20
Chloramphenicol 25 25 10 50%-100% EtOH
Hygromycin 50 25 5 sdH20
Kanamycin 100 50 5 sdH20
Nalidixic Acid 25 25 10 0.2M NaOH
Thiostrepton(e) 50 50 10 100% DMSO or 1:1 DMSO:EtOH
Viomycin 30 30 10 sdH20




E.coli Strain & Plasmid Resistance Markers

E.coli Strain Plasmid / Cosmid E.coli Resistance Marker Plasmid Resistance Marker Temperature Sensitive Replicon
(ts)
BW25113 pIJ790 (λRED) None cat Replication @ 30°C,
Loss of λRED @ 37°C
DH5α None N/A
DH5α BT340 (FLP Recombinase) None cat / blacarb Initial Replication @ 30°C,
Induction of FLP Recombinase & loss of plasmid @ 42°C,
Post recombination growth @37°C
ET12567
[dam-13::Tn9] 		pUZ8002 cat neo
SuperCos 1 neo / blacarb
pIJ773 acc(3)IV




Antibiotic Genes & Information

Antibiotic Name Gene Name Type Common Form
Ampicillin bla Bactericidal Sodium
Apramycin aac(3)IV Aminoglycoside Sulphate
Carbenicillin blacarb Bactericidal DiSodium
Chloramphenicol cat Bacteriostatic
Erythromycin ermE Macrolides
Hygromycin B hph Aminoglycoside
Kanamycin / Neomycin neo Aminoglycoside MonoSulphate
Nalidixic Acid Synthetic Bacteriostatic
Spectinomycin / Streptomycin aadA Aminocyclitol DiHydrochloride Pentahydrate
Tetracyclin Tet Aminoglycoside Hydrochloride
Thiostrepton tsr Aminoglycoside
Viomycin vph Aminoglycoside? Sulphate
Vancomycin B vanB Glycopeptide Hydrochloride



Retrieved from "http://www.openwetware.org/wiki/Streptomyces:Other_Bits/Antibiotic_and_Resistance_Marker_Information"
Personal tools