Streptomyces:Other Bits/Antibiotic and Resistance Marker Information: Difference between revisions
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== | ==Other Bits - Antibiotic & Resistance Marker Information== | ||
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{{Streptomyces}} | {{Streptomyces}} | ||
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[[Category:Material]] | |||
[[Category:Escherichia coli]] | |||
[[Category:Streptomyces]] | |||
[[Category:Antibiotic]] | |||
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<big>'''Antibiotic Concentrations and Usage'''</big> | |||
{|cellspacing=" | {|cellspacing="0" cellpadding="5" rules="rows" frame="hsides" style="width: 770px;" | ||
|- style="text-align:center; | |- style="text-align:center;" valign="top" | ||
! style="width:175px;" | Name || style="width:150px;" | Stock Concentration (mg/ml) || style="width:150px;"| Final Concentration (μg/ml) || style="width:100px;"| Usage in 10mL (μL) || Dissolve in | ! style="width:175px;" | Name || style="width:150px;" | Stock Concentration (mg/ml) || style="width:150px;"| Final Concentration (μg/ml) || style="width:100px;"| Usage in 10mL (μL) || Dissolve in | ||
|- style="text-align:center;" | |- style="text-align:center; border-top: 2px solid black;" | ||
| Ampicillin || 100 || 100 || 10 || sdH<sub>2</sub>0 | | Ampicillin || 100 || 100 || 10 || sdH<sub>2</sub>0 | ||
|- style="text-align:center;" | |- style="text-align:center;" | ||
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| Viomycin || 30 || 30 || 10 || sdH<sub>2</sub>0 | | Viomycin || 30 || 30 || 10 || sdH<sub>2</sub>0 | ||
|} | |} | ||
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<big>'''E.coli Strain & Plasmid Resistance Markers'''</big> | |||
{|cellspacing="0" cellpadding="5" style="width: 770px;" rules="rows" frame="hsides" | |||
|- style="text-align:center;" valign="top" | |||
{|cellspacing=" | ! style="width:130px;" | E.coli Strain || style="width:120px;" | Plasmid / Cosmid || style="width:120px;"| E.coli Resistance Marker || style="width:100px;"| Plasmid Resistance Marker || Temperature Sensitive Replicon <br/>(t<sup>s</sup>) | ||
|- style="text-align:center | |- style="text-align:center; border-top: 2px solid black;" | ||
! style="width:130px;" | E.coli Strain || style="width:120px;" | Plasmid / Cosmid || style="width:120px;"| E.coli Resistance Marker || style="width:100px;"| Plasmid Resistance Marker || Temperature Sensitive Replicon ( | |||
|- style="text-align:center;" | |||
| BW25113 || pIJ790 (λRED) || None || ''cat'' || Replication @ 30°C, <br/>Loss of λRED @ 37°C | | BW25113 || pIJ790 (λRED) || None || ''cat'' || Replication @ 30°C, <br/>Loss of λRED @ 37°C | ||
|- style="text-align:center;" | |- style="text-align:center;" | ||
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| DH5α || BT340 (FLP Recombinase) || None || ''cat'' / ''bla<sub>carb</sub>'' || Initial Replication @ 30°C, <br/>Induction of FLP Recombinase & loss of plasmid @ 42°C, <br/>Post recombination growth @37°C | | DH5α || BT340 (FLP Recombinase) || None || ''cat'' / ''bla<sub>carb</sub>'' || Initial Replication @ 30°C, <br/>Induction of FLP Recombinase & loss of plasmid @ 42°C, <br/>Post recombination growth @37°C | ||
|- style="text-align:center;" | |- style="text-align:center;" | ||
| ET12567 [dam-13::Tn9] || pUZ8002 || ''cat'' || ''neo'' || | | ET12567 <br/>[dam-13::Tn9] || pUZ8002 || ''cat'' || ''neo'' || | ||
|- style="text-align:center;" | |- style="text-align:center;" | ||
| || SuperCos 1 || || ''neo'' / ''bla<sub>carb</sub>'' || | | || SuperCos 1 || || ''neo'' / ''bla<sub>carb</sub>'' || | ||
|- style="text-align:center;" | |- style="text-align:center;" | ||
| || pIJ773 || || ''acc(3)IV'' || | | || pIJ773 || || ''acc(3)IV'' || | ||
|} | |||
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<big>'''Antibiotic Genes & Information'''</big> | |||
{|cellspacing="0" cellpadding="5" style="width: 770px;" rules="rows" frame="hsides" | |||
|- style="text-align:center;" valign="top" | |||
! style="width:225px;" | Antibiotic Name || style="width:125px;" | Gene Name || style="width:125px;"| Type || Common Form | |||
|- style="text-align:center; border-top: 2px solid black;" | |||
| Ampicillin || ''bla'' || Bactericidal || Sodium | |||
|- style="text-align:center;" | |||
| Apramycin || ''aac(3)IV'' || Aminoglycoside || Sulphate | |||
|- style="text-align:center;" | |||
| Carbenicillin || ''bla<sub>carb</sub>'' || Bactericidal || DiSodium | |||
|- style="text-align:center;" | |||
| Chloramphenicol || ''cat'' || Bacteriostatic || | |||
|- style="text-align:center;" | |||
| Erythromycin || ''ermE'' || Macrolides || | |||
|- style="text-align:center;" | |||
| Hygromycin B || ''hph'' || Aminoglycoside || | |||
|- style="text-align:center;" | |||
| Kanamycin / Neomycin || ''neo'' || Aminoglycoside || MonoSulphate | |||
|- style="text-align:center;" | |||
| Nalidixic Acid || Synthetic || Bacteriostatic || | |||
|- style="text-align:center;" | |||
| Spectinomycin / Streptomycin || ''aadA'' || Aminocyclitol || DiHydrochloride Pentahydrate | |||
|- style="text-align:center;" | |||
| Tetracyclin || ''Tet'' || Aminoglycoside || Hydrochloride | |||
|- style="text-align:center;" | |||
| Thiostrepton || ''tsr'' || Aminoglycoside || | |||
|- style="text-align:center;" | |||
| Viomycin || ''vph'' || Aminoglycoside? || Sulphate | |||
|- style="text-align:center;" | |||
| Vancomycin B || ''vanB'' || Glycopeptide || Hydrochloride | |||
|} | |} | ||
Latest revision as of 07:40, 31 August 2007
Other Bits - Antibiotic & Resistance Marker Information
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Antibiotic Concentrations and Usage
E.coli Strain & Plasmid Resistance Markers
Antibiotic Genes & Information
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