Streptomyces:Protocols/PCR: Difference between revisions
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Total volume in a PCR tube - 50µL | Total volume in a PCR tube - 50µL | ||
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{| cellspacing="0" cellpadding="0" | {| cellspacing="0" cellpadding="0" | ||
|- | |- | ||
! style="width: | ! style="width: 200px; text-align: left;"| Promega GoTaq: || style="width: 150px;"| Ingredient || style="width: 100px;"| Stock Conc<sup>n</sup> || style="width: 100px;"| Volume || style="width: 150px;"| Final Conc<sup>n</sup> | ||
|- | |- | ||
| || colspan="4" | | | || colspan="4" | | ||
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| || dH<sub>2</sub>O || N/A || 16.75μL ||N/A | | || dH<sub>2</sub>O || N/A || 16.75μL ||N/A | ||
|- | |- | ||
| | | style="text-align: left;" | Mix and when ready <br/>to start the cycle add: | ||
|- | |- | ||
| || DNA Polymerase || 5u/μL || 0.25μL || 1.25u | | || DNA Polymerase || 5u/μL || 0.25μL || 1.25u | ||
|} | |} | ||
</center> | |||
<br/> | <br/> | ||
< | |||
<center> | |||
{| cellspacing="0" cellpadding="0" | {| cellspacing="0" cellpadding="0" | ||
|- | |- | ||
! style="width: | ! style="width: 200px; text-align: left;"| Roche High Fidelity: || style="width: 150px;"| Ingredient || style="width: 100px;"| Stock Conc<sup>n</sup> || style="width: 100px;"| Volume || style="width: 150px;"| Final Conc<sup>n</sup> | ||
|- | |- | ||
| || colspan="4" | | | || colspan="4" | | ||
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| || dH<sub>2</sub>O || N/A || 16.75μL || N/A | | || dH<sub>2</sub>O || N/A || 16.75μL || N/A | ||
|- | |- | ||
| | | style="text-align: left;" | Mix and when ready <br/>to start the cycle add: | ||
|- | |- | ||
| || DNA Polymerase || 5u/μL || 0.5μL || 2.5u | | || DNA Polymerase || 5u/μL || 0.5μL || 2.5u | ||
|} | |} | ||
</center> | |||
Revision as of 01:31, 10 August 2007
Protocols - PCR
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Polymerase Chain Reaction (PCR)Description
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