Streptomycin

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(Usage Notes)
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==Usage Notes==
==Usage Notes==
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#100 mg/mL stock solution in H<sub>2</sub>O.   
+
#100 mg/mL stock solution in H<sub>2</sub>O.  <br> It is barely soluble in ethanol so you must dissolve it in H<sub>2</sub>O.
#Filter-sterilize with 0.22 &mu;m filter.   
#Filter-sterilize with 0.22 &mu;m filter.   
#Add 1 mL per liter of culture.
#Add 1 mL per liter of culture.

Revision as of 10:02, 24 May 2005

Contents

Mode of Action

Inhibits protein synthesis; binds to 30S ribosomal subunit.

Mechanism of Resistance

Usage Notes

  1. 100 mg/mL stock solution in H2O.
    It is barely soluble in ethanol so you must dissolve it in H2O.
  2. Filter-sterilize with 0.22 μm filter.
  3. Add 1 mL per liter of culture.

References

J. H. Miller. A Short Course in Bacterial Genetics: A Laboratory Manual and Handbook for Escherichia coli and Related Bacteria. Cold Spring Harbor Laboratory Press, 1992.

J. Sambrook and D. W. Russell. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, 3rd edition, 2001.

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