Streptomycin: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
|||
| Line 8: | Line 8: | ||
==Usage Notes== | ==Usage Notes== | ||
#100 mg/mL stock solution in H<sub>2</sub>O. | #100 mg/mL stock solution in H<sub>2</sub>O. <br> It is barely soluble in ethanol so you must dissolve it in H<sub>2</sub>O. | ||
#Filter-sterilize with 0.22 μm filter. | #Filter-sterilize with 0.22 μm filter. | ||
#Add 1 mL per liter of culture. | #Add 1 mL per liter of culture. | ||
Revision as of 08:02, 24 May 2005
Mode of Action
Inhibits protein synthesis; binds to 30S ribosomal subunit.
Mechanism of Resistance
Usage Notes
- 100 mg/mL stock solution in H2O.
It is barely soluble in ethanol so you must dissolve it in H2O. - Filter-sterilize with 0.22 μm filter.
- Add 1 mL per liter of culture.
References
J. H. Miller. A Short Course in Bacterial Genetics: A Laboratory Manual and Handbook for Escherichia coli and Related Bacteria. Cold Spring Harbor Laboratory Press, 1992.
J. Sambrook and D. W. Russell. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, 3rd edition, 2001.
