Streptomycin - Revision history

Reshma P. Shetty at 17:48, 26 July 2007

Reshma P. Shetty — Thu, 26 Jul 2007 17:48:37 GMT

←Older revision		Revision as of 17:48, 26 July 2007
Line 19:		Line 19:

	J. Sambrook and D. W. Russell. ''Molecular Cloning: A Laboratory Manual.'' Cold Spring Harbor Laboratory Press, 3rd edition, 2001.		J. Sambrook and D. W. Russell. ''Molecular Cloning: A Laboratory Manual.'' Cold Spring Harbor Laboratory Press, 3rd edition, 2001.
		+
		+	[[Category:Material]] [[Category:Antibiotic]]

Tk: /* Mode of Action */

Tk — Sat, 07 Jan 2006 16:20:37 GMT

Mode of Action

←Older revision		Revision as of 16:20, 7 January 2006
Line 2:		Line 2:

	Inhibits protein synthesis; binds to 30S ribosomal subunit.		Inhibits protein synthesis; binds to 30S ribosomal subunit.
		+
		+	The rpsL50 gene mutation conveys streptomycin resistance. Resistance is recessive, so the presence of a wild type rpsL gene will convey sensitivity. This allows counter-selection against the presence of the wt rpsL gene. Mutations of wild type genes to convey resistance are common.

	==Mechanism of Resistance==		==Mechanism of Resistance==

Reshma P. Shetty: /* Usage Notes */

Reshma P. Shetty — Tue, 24 May 2005 15:02:29 GMT

Usage Notes

←Older revision		Revision as of 15:02, 24 May 2005
Line 8:		Line 8:
	==Usage Notes==		==Usage Notes==

-	#100 mg/mL stock solution in H<sub>2</sub>O.	+	#100 mg/mL stock solution in H<sub>2</sub>O. <br> It is barely soluble in ethanol so you must dissolve it in H<sub>2</sub>O.
	#Filter-sterilize with 0.22 μm filter.		#Filter-sterilize with 0.22 μm filter.
	#Add 1 mL per liter of culture.		#Add 1 mL per liter of culture.

Reshma P. Shetty at 20:28, 23 May 2005

Reshma P. Shetty — Mon, 23 May 2005 20:28:12 GMT

New page

==Mode of Action==

Inhibits protein synthesis; binds to 30S ribosomal subunit.

==Mechanism of Resistance==

==Usage Notes==

#100 mg/mL stock solution in H<sub>2</sub>O.
#Filter-sterilize with 0.22 μm filter.
#Add 1 mL per liter of culture.

==References==

J. H. Miller. ''A Short Course in Bacterial Genetics: A Laboratory Manual and Handbook for Escherichia coli and Related Bacteria.'' Cold Spring Harbor Laboratory Press, 1992.

J. Sambrook and D. W. Russell. ''Molecular Cloning: A Laboratory Manual.'' Cold Spring Harbor Laboratory Press, 3rd edition, 2001.