Subsoontorn Lab:Research: Difference between revisions
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Our ability to study and utilise microbiota is limited by the lack of tools for precisely perturbing and modulating microbial subpopulations of interest within a heterogenous population. Recently, CRISPR/Cas technology was used for creating antimicrobials with a programmable spectrum of activities.This strategy exploits the fact that CRISPR/Cas system can be designed to break a specific DNA sequence. In a prokaryotic cell without efficient DNA repair, such genomic cleavage often results in cell death. By delivering the designed CRISPR/Cas system to a microbial population one could selectively knockdown a subpopulation whose genomic DNA is targeted. Previous works demonstrated sequence-specific elimination of Escherichia coli and Staphylococcus aureus in mixed populations. Here, we are applying this strategy for targeted elimination of Vibrio harveyi, a pathogenic bacteria in black tiger shrimp and Pacific white shrimp. This project is under collaboration with Dr. Wanilada Rungrassamee (BIOTEC) and Prof. Dr. Jim Haseloff (University of Cambridge, UK) | Our ability to study and utilise microbiota is limited by the lack of tools for precisely perturbing and modulating microbial subpopulations of interest within a heterogenous population. Recently, CRISPR/Cas technology was used for creating antimicrobials with a programmable spectrum of activities.This strategy exploits the fact that CRISPR/Cas system can be designed to break a specific DNA sequence. In a prokaryotic cell without efficient DNA repair, such genomic cleavage often results in cell death. By delivering the designed CRISPR/Cas system to a microbial population one could selectively knockdown a subpopulation whose genomic DNA is targeted. Previous works demonstrated sequence-specific elimination of Escherichia coli and Staphylococcus aureus in mixed populations. Here, we are applying this strategy for targeted elimination of Vibrio harveyi, a pathogenic bacteria in black tiger shrimp and Pacific white shrimp. This project is under collaboration with Dr. Wanilada Rungrassamee (BIOTEC) and Prof. Dr. Jim Haseloff (University of Cambridge, UK) | ||
http://www.stanforddaily.com/2017/02/02/stanford-students-explore-bio-innovation-with-biome/ Stanford Daily] | http://www.stanforddaily.com/2017/02/02/stanford-students-explore-bio-innovation-with-biome/ | Stanford Daily] | ||
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Revision as of 20:42, 9 May 2017
Current Research
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Our ability to study and utilise microbiota is limited by the lack of tools for precisely perturbing and modulating microbial subpopulations of interest within a heterogenous population. Recently, CRISPR/Cas technology was used for creating antimicrobials with a programmable spectrum of activities.This strategy exploits the fact that CRISPR/Cas system can be designed to break a specific DNA sequence. In a prokaryotic cell without efficient DNA repair, such genomic cleavage often results in cell death. By delivering the designed CRISPR/Cas system to a microbial population one could selectively knockdown a subpopulation whose genomic DNA is targeted. Previous works demonstrated sequence-specific elimination of Escherichia coli and Staphylococcus aureus in mixed populations. Here, we are applying this strategy for targeted elimination of Vibrio harveyi, a pathogenic bacteria in black tiger shrimp and Pacific white shrimp. This project is under collaboration with Dr. Wanilada Rungrassamee (BIOTEC) and Prof. Dr. Jim Haseloff (University of Cambridge, UK) http://www.stanforddaily.com/2017/02/02/stanford-students-explore-bio-innovation-with-biome/ | Stanford Daily]
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Past Research
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