Suggs:Protocols

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===MSC media preparation===
===MSC media preparation===
 +
{| border="1" cellpadding="5"
 +
!<u>Item</u> || <u>[Stock]</u> !! <u>[Media]</u> !! <u>Amt/500mL</u> !! <u>Store</u>
 +
|-
 +
!Dexamethasone
 +
|.25 nM
 +
|.05 nM
 +
|100 uL
 +
|(-80)
 +
|-
 +
!DMEM
 +
|1X
 +
|.6X
 +
|300 mL
 +
|(4)
 +
|-
 +
!EGF
 +
|10 ug/mL
 +
|10 ng/mL
 +
|500 uL
 +
|(-80)
 +
|-
 +
!FCS
 +
|100%
 +
|1%
 +
|10 mL
 +
|(-20)
 +
|-
 +
!ITS
 +
|100%
 +
|1%
 +
|5 mL
 +
|(4)
 +
|-
 +
!LA-BSA
 +
|100 mg/mL
 +
|1 mg/mL
 +
|5 mL
 +
|(4)
 +
|-
 +
!L-ascorbic acid salt
 +
|10 nM
 +
|.1 nM
 +
|5 mL
 +
|(-20)
 +
|-
 +
!L-glutamine
 +
|200 nM
 +
|2 nM
 +
|5 mL
 +
|(-20)
 +
|-
 +
!MCDB 201 Medium
 +
|1X
 +
|.4X
 +
|200 mL
 +
|(4)
 +
|-
 +
!Pen/strep
 +
|100X
 +
|1X
 +
|5 mL
 +
|(-20)
 +
|-
 +
!PDGF
 +
|10 ug/mL
 +
|10 ng/mL
 +
|500 uL
 +
|(-80)
 +
|-
 +
|}
 +
 +
Filter at end.
===MSC subculturing===
===MSC subculturing===
===MSC freezing===
===MSC freezing===

Revision as of 16:43, 9 September 2005

MSC media preparation

Item [Stock] [Media] Amt/500mL Store
Dexamethasone .25 nM .05 nM 100 uL (-80)
DMEM 1X .6X 300 mL (4)
EGF 10 ug/mL 10 ng/mL 500 uL (-80)
FCS 100% 1% 10 mL (-20)
ITS 100% 1% 5 mL (4)
LA-BSA 100 mg/mL 1 mg/mL 5 mL (4)
L-ascorbic acid salt 10 nM .1 nM 5 mL (-20)
L-glutamine 200 nM 2 nM 5 mL (-20)
MCDB 201 Medium 1X .4X 200 mL (4)
Pen/strep 100X 1X 5 mL (-20)
PDGF 10 ug/mL 10 ng/mL 500 uL (-80)

Filter at end.

MSC subculturing

MSC freezing

Retrieved from "http://www.openwetware.org/wiki/Suggs:Protocols"
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