Suggs:Protocols
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MSC media preparation
| Item | [Stock] | [Media] | Amt/500mL | Store |
|---|---|---|---|---|
| Dexamethasone | .25 nM | .05 nM | 100 uL | (-80) |
| DMEM | 1X | .6X | 300 mL | (4) |
| EGF | 10 ug/mL | 10 ng/mL | 500 uL | (-80) |
| FCS | 100% | 1% | 10 mL | (-20) |
| ITS | 100% | 1% | 5 mL | (4) |
| LA-BSA | 100 mg/mL | 1 mg/mL | 5 mL | (4) |
| L-ascorbic acid salt | 10 nM | .1 nM | 5 mL | (-20) |
| L-glutamine | 200 nM | 2 nM | 5 mL | (-20) |
| MCDB 201 Medium | 1X | .4X | 200 mL | (4) |
| Pen/strep | 100X | 1X | 5 mL | (-20) |
| PDGF | 10 ug/mL | 10 ng/mL | 500 uL | (-80) |
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