Suggs:Protocols

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MSC media preparation

Item [Stock] [Media] Amt/500mL Store
Dexamethasone .25 nM .05 nM 100 uL (-80)
DMEM 1X .6X 300 mL (4)
EGF 10 ug/mL 10 ng/mL 500 uL (-80)
FCS 100% 1% 10 mL (-20)
ITS 100% 1% 5 mL (4)
LA-BSA 100 mg/mL 1 mg/mL 5 mL (4)
L-ascorbic acid salt 10 nM .1 nM 5 mL (-20)
L-glutamine 200 nM 2 nM 5 mL (-20)
MCDB 201 Medium 1X .4X 200 mL (4)
Pen/strep 100X 1X 5 mL (-20)
PDGF 10 ug/mL 10 ng/mL 500 uL (-80)

Filter at end.

MSC subculturing

MSC freezing

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