Suggs:Protocols
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MSC media preparation
Item | [Stock] | [Media] | Amt/500mL | Store |
---|---|---|---|---|
Dexamethasone | .25 nM | .05 nM | 100 uL | (-80) |
DMEM | 1X | .6X | 300 mL | (4) |
EGF | 10 ug/mL | 10 ng/mL | 500 uL | (-80) |
FCS | 100% | 1% | 10 mL | (-20) |
ITS | 100% | 1% | 5 mL | (4) |
LA-BSA | 100 mg/mL | 1 mg/mL | 5 mL | (4) |
L-ascorbic acid salt | 10 nM | .1 nM | 5 mL | (-20) |
L-glutamine | 200 nM | 2 nM | 5 mL | (-20) |
MCDB 201 Medium | 1X | .4X | 200 mL | (4) |
Pen/strep | 100X | 1X | 5 mL | (-20) |
PDGF | 10 ug/mL | 10 ng/mL | 500 uL | (-80) |
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