Swartz:Protocols: Difference between revisions
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=Protocols= | =Protocols= | ||
==Lab Ordering== | |||
*[https://docs.google.com/spreadsheet/viewform?formkey=dGVtSVdMVnpiWWtGbU9FVjF6TUhPb2c6MQ#gid=0 USE THIS FORM] | |||
==Cloning== | ==Cloning== | ||
:*[https://docs.google.com/document/d/1KHeoV1WNDpWqD-90k0X5MtVM1naTK5_81uctSkKF5z0/edit Swartz Lab Molecular Biology Handbook] | :*[https://docs.google.com/document/d/1KHeoV1WNDpWqD-90k0X5MtVM1naTK5_81uctSkKF5z0/edit Swartz Lab Molecular Biology Handbook] | ||
:*[https://docs.google.com/spreadsheet/ccc?key=0AuGtz3V56Y9xdGQyaEUtM1p1TTh1dmQtZHhLUWVpclE&pli=1#gid=5 Restriction Enzyme Library] | :*[https://docs.google.com/spreadsheet/ccc?key=0AuGtz3V56Y9xdGQyaEUtM1p1TTh1dmQtZHhLUWVpclE&pli=1#gid=5 Restriction Enzyme Library] | ||
:*[[Gibson_Assembly]] | |||
'''DNA Design''' | '''DNA Design''' | ||
:*[[Swartz:Protocols/Primer Design|Primer Design]] | :*[[Swartz:Protocols/Primer Design|Primer Design]] | ||
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:*[https://docs.google.com/document/d/17lf6hG6u25vGYEW3y1CxoGyKHr-yJv9LbZeoYSGOw04/edit Gel Purification/PCR Cleanup] | :*[https://docs.google.com/document/d/17lf6hG6u25vGYEW3y1CxoGyKHr-yJv9LbZeoYSGOw04/edit Gel Purification/PCR Cleanup] | ||
:*[[/Column_Regeneration|MaxiPrep Column Regeneration]] | :*[[/Column_Regeneration|MaxiPrep Column Regeneration]] | ||
:*[[Genomic DNA Extraction|Genomic DNA Extraction]] | |||
'''Expression Templates''' | '''Expression Templates''' | ||
:* | :* | ||
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'''Organisms''' | '''Organisms''' | ||
:*[[Transforming chemically competent cells|E.coli Transformation]] | :*[[Transforming chemically competent cells|E.coli Transformation]] | ||
:*[[/Cloning| Other Cloning Protocols]] | :*[[/Cloning| Other Cloning Protocols]] | ||
:*[[Making_a_long_term_stock_of_bacteria| Creating an E. coli glycerol stock]] | :*[[Making_a_long_term_stock_of_bacteria| Creating an E. coli glycerol stock]] | ||
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:*[[/otDNA|Orthogonal tDNA template prep]] | :*[[/otDNA|Orthogonal tDNA template prep]] | ||
:*[[/Protein_gels|Running a protein gel]] | :*[[/Protein_gels|Running a protein gel]] | ||
**[[S30_buffer]] | |||
==Protein Purification== | ==Protein Purification== | ||
Latest revision as of 13:57, 12 October 2012
| Research | Papers | People | Contact |
Getting settled in the Swartz Lab
Protocols
Lab Ordering
Cloning
DNA Design
Gene Synthesis
Expression Templates
Plasmids
Organisms
