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== | =Protocols= | ||
==Lab Ordering== | |||
*[https://docs.google.com/spreadsheet/viewform?formkey=dGVtSVdMVnpiWWtGbU9FVjF6TUhPb2c6MQ#gid=0 USE THIS FORM] | |||
==Cloning== | |||
:*[https://docs.google.com/document/d/1KHeoV1WNDpWqD-90k0X5MtVM1naTK5_81uctSkKF5z0 Swartz Lab Molecular Biology Handbook] | :*[https://docs.google.com/document/d/1KHeoV1WNDpWqD-90k0X5MtVM1naTK5_81uctSkKF5z0/edit Swartz Lab Molecular Biology Handbook] | ||
:*[https://docs.google.com/spreadsheet/ccc?key=0AuGtz3V56Y9xdGQyaEUtM1p1TTh1dmQtZHhLUWVpclE&pli=1#gid=5 Restriction Enzyme Library] | :*[https://docs.google.com/spreadsheet/ccc?key=0AuGtz3V56Y9xdGQyaEUtM1p1TTh1dmQtZHhLUWVpclE&pli=1#gid=5 Restriction Enzyme Library] | ||
:*[[Gibson_Assembly]] | |||
'''DNA Design''' | '''DNA Design''' | ||
:*[[Swartz:Protocols/Primer Design|Primer Design]] | :*[[Swartz:Protocols/Primer Design|Primer Design]] | ||
| Line 15: | Line 18: | ||
:*[[Swartz:Protocols/Gene Synthesis|Gene Synthesis]] | :*[[Swartz:Protocols/Gene Synthesis|Gene Synthesis]] | ||
:*[[Swartz:Protocols/Restriction Cloning|Restriction Enzyme Digestion/Ligations]] | :*[[Swartz:Protocols/Restriction Cloning|Restriction Enzyme Digestion/Ligations]] | ||
:*[https://docs.google.com/document/d/ | :*[[Swartz:Protocols/Quikchange|Quikchange]] | ||
:*[https://docs.google.com/document/d/17lf6hG6u25vGYEW3y1CxoGyKHr-yJv9LbZeoYSGOw04/edit Gel Purification/PCR Cleanup] | |||
:*[[/Column_Regeneration|MaxiPrep Column Regeneration]] | |||
:*[[Genomic DNA Extraction|Genomic DNA Extraction]] | |||
'''Expression Templates''' | '''Expression Templates''' | ||
:* | |||
'''Plasmids''' | '''Plasmids''' | ||
:*[[Miniprep/Qiagen kit|Plasmid Miniprep]] | :*[[Miniprep/Qiagen kit|OWW Plasmid Miniprep]] or [https://docs.google.com/document/d/1kX8-atjv8nRfLn1346UDbteZq_5_a2lVSzmtqU_XBYk/edit | Swartz Lab Version] | ||
:*[[/Maxiprep|Plasmid Maxiprep]] | |||
'''Organisms''' | '''Organisms''' | ||
:*[[Transforming chemically competent cells|E.coli Transformation]] | :*[[Transforming chemically competent cells|E.coli Transformation]] | ||
:*[[/Cloning| Other Cloning Protocols]] | :*[[/Cloning| Other Cloning Protocols]] | ||
:*[[Making_a_long_term_stock_of_bacteria| Creating an E. coli glycerol stock]] | |||
==Cell Free Protein Synthesis== | |||
:*[[/Linear_DNA_Template|Preparing linear DNA template for CFPS]] | |||
:*[[/1L_extract_prep|1L Extract Prep]] | |||
:*[[/Cell_Free|Cell Free Protein Synthesis]] | :*[[/Cell_Free|Cell Free Protein Synthesis]] | ||
:*[ | :*[[/Autoradiogram|How to do an Autoradiogram]] | ||
:*[[/Western|Western Blotting]] | |||
:*[[/HD_Extract_Prep|High Density Fermentation]] | |||
:*[[/otDNA|Orthogonal tDNA template prep]] | |||
:*[[/Protein_gels|Running a protein gel]] | |||
**[[S30_buffer]] | |||
===Assays== | ==Protein Purification== | ||
:*[[/Purification_of_His-tagged_proteins|His-Tag Purification]] | |||
:*[[/Ribosome_purification|Ribosome purification]] | |||
==Assays== | |||
:*[[Nanodrop]] | |||
:*[[DNA_Quantification | Using the spec for DNA Quantification]] | |||
:*[[/Mass_spec| Sample prep for MS]] | |||
:*[[/Ribosome_binding| Spin assay for ribsome binding]] | |||
:*[[/HPLC|HPLC]] | |||
:*[[Free_Sulfhydryl_Determination| Ellman's Reagent Test]] | |||
:*[[Swartz:Protocols/Cytochrome C|Cytochrome C ]] | :*[[Swartz:Protocols/Cytochrome C|Cytochrome C ]] | ||
:*[[Swartz:Protocols/Methyl Viologen|Methyl Viologen]] | :*[[Swartz:Protocols/Methyl Viologen|Methyl Viologen]] | ||
:*[[Swartz:Protocols/H2 Production (GC)|H2 Production (GC)]] | :*[[Swartz:Protocols/H2 Production (GC)|H2 Production (GC)]] | ||
==Miscellaneous== | |||
:*[https://docs.google.com/document/d/1nlZCY_zG3eyBWw3DReVQHMm5oV88eX7cJpdavRdB2cw/edit Anaerobic Protein Production] | :*[https://docs.google.com/document/d/1nlZCY_zG3eyBWw3DReVQHMm5oV88eX7cJpdavRdB2cw/edit Anaerobic Protein Production in E.coli] | ||
:*[[/A20_cells|Cell culture for A20 B-cells]] | |||
==Solutions== | ==Solutions== | ||
:*[[/Buffers|Buffer Recipes]] | :*[[/Buffers|Buffer Recipes]] | ||
[[Category:Swartz_Protocols]] | |||
Latest revision as of 13:57, 12 October 2012
| Research | Papers | People | Contact |
Getting settled in the Swartz Lab
Protocols
Lab Ordering
Cloning
DNA Design
Gene Synthesis
Expression Templates
Plasmids
Organisms
