Swartz:Protocols: Difference between revisions
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=Protocols= | |||
==Cloning== | |||
:*[https://docs.google.com/document/d/1KHeoV1WNDpWqD-90k0X5MtVM1naTK5_81uctSkKF5z0/edit Swartz Lab Molecular Biology Handbook] | :*[https://docs.google.com/document/d/1KHeoV1WNDpWqD-90k0X5MtVM1naTK5_81uctSkKF5z0/edit Swartz Lab Molecular Biology Handbook] | ||
:*[https://docs.google.com/spreadsheet/ccc?key=0AuGtz3V56Y9xdGQyaEUtM1p1TTh1dmQtZHhLUWVpclE&pli=1#gid=5 Restriction Enzyme Library] | :*[https://docs.google.com/spreadsheet/ccc?key=0AuGtz3V56Y9xdGQyaEUtM1p1TTh1dmQtZHhLUWVpclE&pli=1#gid=5 Restriction Enzyme Library] | ||
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:*[[/Cloning| Other Cloning Protocols]] | :*[[/Cloning| Other Cloning Protocols]] | ||
==Cell Free Protein Synthesis== | |||
:*[[/Linear_DNA_Template|Preparing linear DNA template for CFPS]] | :*[[/Linear_DNA_Template|Preparing linear DNA template for CFPS]] | ||
:*[[/1L_extract_prep|1L Extract Prep]] | :*[[/1L_extract_prep|1L Extract Prep]] | ||
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:*[[/Western|Western Blotting]] | :*[[/Western|Western Blotting]] | ||
==Protein Purification== | |||
:*[[/Purification_of_His-tagged_proteins|His-Tag Purification]] | :*[[/Purification_of_His-tagged_proteins|His-Tag Purification]] | ||
:*[[/Ribosome_purification|Ribosome purification]] | :*[[/Ribosome_purification|Ribosome purification]] | ||
==Assays== | |||
:*[[Nanodrop]] | :*[[Nanodrop]] | ||
:*[[/Mass_spec| Sample prep for MS]] | :*[[/Mass_spec| Sample prep for MS]] | ||
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:*[[Swartz:Protocols/H2 Production (GC)|H2 Production (GC)]] | :*[[Swartz:Protocols/H2 Production (GC)|H2 Production (GC)]] | ||
==Miscellaneous== | |||
:*[https://docs.google.com/document/d/1nlZCY_zG3eyBWw3DReVQHMm5oV88eX7cJpdavRdB2cw/edit Anaerobic Protein Production] | :*[https://docs.google.com/document/d/1nlZCY_zG3eyBWw3DReVQHMm5oV88eX7cJpdavRdB2cw/edit Anaerobic Protein Production] | ||
:*[[/A20_cells|Cell culture for A20 B-cells]] | :*[[/A20_cells|Cell culture for A20 B-cells]] | ||
Revision as of 00:30, 15 May 2012
| Research | Papers | People | Contact |
Getting settled in the Swartz Lab
Protocols
Cloning
DNA Design
Gene Synthesis
Expression Templates
Plasmids
Organisms
