Swartz:Protocols/Column Regeneration: Difference between revisions
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m (New page: https://docs.google.com/document/d/19E-F4kt27GzKnOS9kxA2BqQl6a7mDHYxDjyiQtsPrno/edit ==Recycling Qiagen Columns== :From Qiagen Buffers The blue and purple Qiagen columns are identica...) |
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References: PMID 17373483 [http://www.bio.davidson.edu/courses/Molbio/Protocols/QIAQuick_recycle.html Davidson Column Recycling instructions] | References: PMID 17373483 [http://www.bio.davidson.edu/courses/Molbio/Protocols/QIAQuick_recycle.html Davidson Column Recycling instructions] | ||
[[Category:Swartz_Protocols]] |
Latest revision as of 16:46, 28 May 2012
https://docs.google.com/document/d/19E-F4kt27GzKnOS9kxA2BqQl6a7mDHYxDjyiQtsPrno/edit
Recycling Qiagen Columns
- From Qiagen Buffers
The blue and purple Qiagen columns are identical in formulation. They are interchangeable and can be reused multiple times after treatment with hydrochloric acid, re-equilibration, and wash. Very likely this protocol can be used with other similar columns. Unused columns can be cheaply purchased in bulk from Epoch Biolabs.
The reuse protocol is:
- Save the collection tubes and columns after elution of DNA
- Fill the column with 700 μl of 1 M HCl
- Cap and store in an airtight container for at least 24 hours (less than a month)
- Wash the columns and collection tubes in a large beaker of water
- Assemble the column and collection tube and wash the column with 700 μl of DI water, discarding the water
- Repeat
- Fill the column with 700 μl of buffer QBT and spin down, discarding the buffer
- Place the columns in an airtight plastic bag for storage
- Wash the collection tubes, air dry, and store them for reuse
References: PMID 17373483 Davidson Column Recycling instructions