http://www.openwetware.org/index.php?title=SynBERC:Automated_cloning_brainstorming&feed=atom&action=history 2013-05-24T18:37:43Z Revision history for this page on the wiki MediaWiki 1.13.2 http://www.openwetware.org/index.php?title=SynBERC:Automated_cloning_brainstorming&diff=150729&oldid=prev 2007-09-13T16:57:30Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 16:57, 13 September 2007</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 10:</td> <td colspan="2" class="diff-lineno">Line 10:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>practical outcomes for each of these sessions as part of the</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>practical outcomes for each of these sessions as part of the</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>brainstorming sessions?&quot;</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>brainstorming sessions?&quot;</div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">==Meta questions==</ins></div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">#Does SynBERC want to make investments in automated cloning?</ins></div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">#How could SynBERC best contribute to this endeavors?</ins></div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">#If we do want to work this initiative, how do we want to structure the investment? (Personnel, consulting with companies, research project in a lab?)</ins></div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">#What is our vision for the outcome of a SynBERC investment in automated cloning?</ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Practical outcomes==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Practical outcomes==</div></td></tr> <!-- diff generator: internal 2013-05-24 18:37:43 --> </table>

Reshma P. Shetty http://www.openwetware.org/index.php?title=SynBERC:Automated_cloning_brainstorming&diff=150690&oldid=prev 2007-09-13T16:02:55Z

<p>New page: from Kevin: &quot;I was talking with Adam Arkin, who runs a DOE:GTL program and is pretty good at executing large-scale, center-based research projects like SynBERC. For the cloning and regist...</p> <p><b>New page</b></p><div>from Kevin:<br /> <br /> &quot;I was talking with Adam Arkin, who runs a DOE:GTL program and is pretty<br /> good at executing large-scale, center-based research projects like<br /> SynBERC. For the cloning and registry discussions, Adam suggested that<br /> we first agree on what decisions we are going to make before we start<br /> hashing out the issues. The idea is that it's better to settle up-front<br /> on what we want to emerge from the discussion before we get bogged down<br /> in technical complexities. Could the SLC/student sessions suggest some<br /> practical outcomes for each of these sessions as part of the<br /> brainstorming sessions?&quot;<br /> <br /> ==Practical outcomes==<br /> *'''[[User:Jason R. Kelly|Jason R. Kelly]] 12:02, 13 September 2007 (EDT):'''Answers to some of these questions would be nice outcome I think:<br /> *Where should we focus our efforts? Standard BB cloning, BamI/BglI, BBQuick, OE-PCR w/ custom primers, give up and let direct synthesis do it, etc...<br /> *Can we get some funding for this?<br /> *Can we partner with a synthesis company? Bangalore Genie? etc?<br /> *What is the acceptable cost per assembly? For instance if we would pay $1000 an assembly we could just hire technicians to do BB assembly by hand all day for us -- or I'd do it ;)</div>

Jason R. Kelly