SynBERC:COG/October 2007 Meeting
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Notes
Agenda
What are the goals for COG?
Proposed based on SynBERC meeting / conversations since:
- Stay up to date with current automated cloning research projects taking place in SynBERC labs
- Get the ball rolling for solving the automated cloning problem in the short term
Research Updates
- 1/2 the meeting?
- Brief updates from each of the groups? one longer presentation each month about one project?
- Something else?
Getting the ball rolling for solving automated cloning
- Define the problem we want solved
- Specify the possible solutions
- Evaluate / Act on solutions
Define problem(s)
- Combinatorial libraries of assembled parts (Wendall, UCSF iGEM team project)
- Automation of standard BioBrick Assembly (Registry)
- Automation of assembly independent of BBs (Synth companies?)
Specify Possible Solutions
- Decentralized approach
- We make cloning easier by using some automation combined with "normal" individual cloning approaches. For example, each lab buys a Qiacube to partially automate minipreps and restriction digests. Then ligations and transformations are done by hand at the bench.
- Centralized academic facility
- We find the resources to start an assembly facility similar to how sequencing facilities are set up on campus
- Outsource to DNA synthesis company
- We work with a DNA synthesis company so that they can offer commercial automated cloning services. How do we ensure that such a partnership wouldn't fall apart as soon as a big synthesis order comes along? Automated cloning may be too small a market and/or too large a problem for DNA synthesis companies to tackle right now?
Evaluate / Act on Solutions
- Registry hires more staff to do process engineering
- Trial QIAcube / other "cheap" robots for distributed approach
- Figure out how big an assembly order iGEM + labs using parts could put together for a Synth company (George suggestion)
- Go talk to Synth companies see if they'll do this.
- Plot the cost of synth vs. assembly over time for particular length scales (drew's suggestion)
Meeting Frequency
- Monthly?
- Any weekday's particularly bad for certain groups? (Weds at MIT)