http://www.openwetware.org/index.php?title=SynBERC:Drugs&feed=atom&action=history 2013-05-24T10:47:42Z Revision history for this page on the wiki MediaWiki 1.13.2 http://www.openwetware.org/index.php?title=SynBERC:Drugs&diff=70099&oldid=prev 2006-09-13T00:01:43Z

<p><span class="autocomment">Objecive: Microbial synthesis of natural and unnatural organic compounds</span></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 00:01, 13 September 2006</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 1:</td> <td colspan="2" class="diff-lineno">Line 1:</td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>==<del class="diffchange diffchange-inline">Objecive</del>:&nbsp; Microbial synthesis of natural and unnatural organic compounds==</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>==<ins class="diffchange diffchange-inline">Objective</ins>:&nbsp; Microbial synthesis of natural and unnatural organic compounds==</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>This testbed is broken into two parts:</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>This testbed is broken into two parts:</div></td></tr> <!-- diff generator: internal 2013-05-24 10:47:42 --> </table>

Geochurch http://www.openwetware.org/index.php?title=SynBERC:Drugs&diff=70096&oldid=prev 2006-09-12T23:49:02Z

<p></p> <p><b>New page</b></p><div>==Objecive: Microbial synthesis of natural and unnatural organic compounds==<br /> <br /> This testbed is broken into two parts:<br /> <br /> #Microbial synthesis of terpene indole alkaloids (TIAs), plant natural products<br /> #''de novo'' design of biosynthetic pathways<br /> <br /> ==Parts==<br /> * malic acid to malic semialdehyde converting enzyme<br /> <br /> * 7-deoxyloganin synthetic enzymes (from glucose + GPP)<br /> <br /> * vinblastine/vincristine transporter<br /> <br /> ==Devices==<br /> ====Precursor Amplifiers====<br /> * Over-expression of rate-limiting enzyme(s) for precursor formation<br /> <br /> * Simple expression device (promoter, RBS, ORF (protein coding), terminator)<br /> <br /> * Amp needs: tryptophan, geranyl diphoshpate (GPP), malic acid<br /> <br /> ====Multi-cistronic/Multi-genic Biosynthetic Devices====<br /> * Multiple ORFs with individual promoter/terminator pairs or as multi-cistronic operons<br /> <br /> ====Metabolite Sensors====<br /> Needed to rapidly evaluate success in achieving synthesis goals.<br /> <br /> * secologanin<br /> * strictosidine<br /> * 3,4-dihydroxbutyric acid<br /> <br /> ====Metabolite-controlled Biosynthetic Devices====<br /> * Includes parts in multi-cistronic/multi-genic device<br /> <br /> * Metabolite-responsive expression (transcriptional control -- same switches can be used in sensors and control devices)<br /> ** secolaganin<br /> ** strictosidine<br /> ** 3,4-dihydroxybutyric acid<br /> <br /> ====Extracellular/Surface-associated Biosynthetic Device====<br /> * Surface-anchoring protein part<br /> * promoter, RBS, ORF, terminator<br /> <br /> ==Chassis==<br /> * Precursor supply<br /> ** tryptophan<br /> ** geranyl diphosphate (GPP)<br /> ** malic acid<br /> <br /> * Free glucose (PTS-independent) transport</div>

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