SynBERC:MIT/Calendar/2007-8-8

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Anyone in the synthetic biology community is welcome to attend.

Wednesday August 8, 2007 at 12:30pm EST

32-D463, MIT

Topic of discussion

Jason Kelly will be leading a discussion on how do we make BioBricks more useful for academic research labs. In this discussion, we will also be generating a list of "wanted" BioBrick parts.

Wanted parts

• Expression biobrick plasmids
  • T7 promoter - may have patent issues but it has been tried and tested.
  • T3
  • SP6
  • T7 RNA polymerase containing strains
  • also T3, SP6 What are the patent issues on these?

• Popular plasmids
  • Any requests through the SynBERC BB plasmid project? (Collin)

• xre protein, promoters (subtilis)

• antibiotic cassettes
  • Apramycin
  • Trimethoprim

• Transposase genes

• Plasmid origins
  • R6, P1, F, colE1

• chomosomal integration fragments
  • choice of locations on the chromosome

• cell killing enzymes
  • VSVG

• protein export tags
  • phoA
• protein purification tags, antibody epitopes
  • FLAG
  • 6HIS
  • Strep
  • GST
  • Chitin binding
  • MBP

• protein solubility
  • maltose binding protein

• protein splicing domains

• surface display proteins
  • LPP/OmpA
  • Neisseria IgA1

• New fluorescent proteins
  • EBFP2 blue
  • new red monomers

• luminescent reporters
  • renilla
  • photuris
  • bacterial (Photorhabdus)

• Promoters in reverse direction

• Parts to enable custom insertions in the middle of devices (e.g. a set of alternate restriction sites that are also kept out of BBs (and BB plasmids) when possible to support using them for editing)

• Add your wanted part here ...

Live Notes

Rocking the notetaking:

• Audience: Asking about creating a 5-gene operon, something to do with the PZE system, interested in using BB methods to build permutations of the transcription logic

• Audience: GST tags are key for surface plasmon resonance

Question: how do people keep track of plasmids and their constructs?

Problem: right now people do not have a way to import or export annotated sequence files from the registry.

Jason: one of the most useful functions of the Registry is documentation and archival of parts.

The Sauer lab just uses a notepad to keep track of plasmids.