SynBERC:MIT/Calendar/2007-8-8: Difference between revisions
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The Sauer lab just uses a notepad to keep track of plasmids. | The Sauer lab just uses a notepad to keep track of plasmids. | ||
We need better education about the ease of assembling pieces of DNA via BioBricks assembly. This should also involve statistics on assembly success rate. |
Revision as of 10:29, 8 August 2007
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Anyone in the synthetic biology community is welcome to attend.
Wednesday August 8, 2007 at 12:30pm EST
32-D463, MIT
Topic of discussion
Jason Kelly will be leading a discussion on how do we make BioBricks more useful for academic research labs. In this discussion, we will also be generating a list of "wanted" BioBrick parts.
Wanted parts
- Expression biobrick plasmids
- T7 promoter - may have patent issues but it has been tried and tested.
- T3
- SP6
- T7 RNA polymerase containing strains
- also T3, SP6 What are the patent issues on these?
- Popular plasmids
- Any requests through the SynBERC BB plasmid project? (Collin)
- xre protein, promoters (subtilis)
- antibiotic cassettes
- Apramycin
- Trimethoprim
- Transposase genes
- Plasmid origins
- R6, P1, F, colE1
- chomosomal integration fragments
- choice of locations on the chromosome
- cell killing enzymes
- VSVG
- protein export tags
- phoA
- protein purification tags, antibody epitopes
- FLAG
- 6HIS - Sauer lab
- Strep
- GST
- Chitin binding
- MBP
- S tags (is this the same as strep?)
- protein solubility
- maltose binding protein
- protein splicing domains
- surface display proteins
- LPP/OmpA
- Neisseria IgA1
- New fluorescent proteins
- EBFP2 blue
- new red monomers
- luminescent reporters
- renilla
- photuris
- bacterial (Photorhabdus)
- Promoters in reverse direction
- Parts to enable custom insertions in the middle of devices (e.g. a set of alternate restriction sites that are also kept out of BBs (and BB plasmids) when possible to support using them for editing)
- the pZE modular plasmid system - Stephanopoulous lab
- Add your wanted part here ...
Live Notes
Rocking the notetaking:
- Audience: Asking about creating a 5-gene operon, something to do with the PZE system, interested in using BB methods to build permutations of the transcription logic
- Audience: GST tags are key for surface plasmon resonance
Question: how do people keep track of plasmids and their constructs?
Problem: right now people do not have a way to import or export annotated sequence files from the registry.
Jason: one of the most useful functions of the Registry is documentation and archival of parts.
The Sauer lab just uses a notepad to keep track of plasmids.
We need better education about the ease of assembling pieces of DNA via BioBricks assembly. This should also involve statistics on assembly success rate.