SynBERC:Seminar series/Ichiro Matsumura: Difference between revisions
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'''Acinetobacter baylyi ADP1 as a chassis for the directed evolution of genes and genomes'''<br> | '''Acinetobacter baylyi ADP1 as a chassis for the directed evolution of genes and genomes'''<br> | ||
'''Ichiro Matsumura, Associate Professor of Biochemistry, Emory University School of Medicine''' | '''Ichiro Matsumura, Associate Professor of Biochemistry, Emory University School of Medicine''' | ||
Follow link to see video: http://vimeo.com/4367036 | |||
Wedesday, April 22, 2009<br> | Wedesday, April 22, 2009<br> | ||
noon-1 pm ET<br> | noon-1 pm ET<br> | ||
Latest revision as of 21:55, 11 May 2009
Acinetobacter baylyi ADP1 as a chassis for the directed evolution of genes and genomes
Ichiro Matsumura, Associate Professor of Biochemistry, Emory University School of Medicine
Follow link to see video: http://vimeo.com/4367036
Wedesday, April 22, 2009
noon-1 pm ET
RM 449 (Kiva/Patil,) Stata Center (Building 32,) MIT
Synthetic biologists generally build upon the Escherichia coli chassis, which usually necessitates a life of cloning. Acinetobacter baylyi sp. ADP1 offers two significant advantages over E. coli: natural competence, and efficient homologous recombination. I will explain how these natural advantages obviate cloning and streamline the directed evolution of genes and genomes. I will also describe the engineering of a broad host range expression vector pBAV1K (Biobrick Accepting Vector) that replicates and produces proteins in a wide variety of gram negative and gram positive bacteria. These tools enable the facile laboratory evolution of genes and chromosomes, without restriction digests or ligation reactions.
