Synthetic Biology:Vectors/Inventory

See the vector nomenclature for an explanation of what the vector names mean.

pSB1* Plasmids

pSB1A1

• constructed by Tom Knight

pSB1A2-P1010

• (formerly known as pSB1A2-1)
• derived from pSB1A1
• constructed by Leon Chan

pSB1A3-P1010

• (formerly known as pSB1A3-1 or pSB104.2)
• purpose: base plasmid used to insert biobricks parts
• derived from pSB1A2-1
• constructed by Reshma

pSB1AT3-P1010

• (formerly known as pSB1AT3-1 or pSB204.3 or pSB3.1.7.1)
• purpose: high copy number plasmid to be used in the three antibiotic selection assembly technique.
• derived from pSB1A3-1
• constructed by Reshma

pSB1AK3-P1010

• (formerly known as pSB1AK3-1 or pSB304.3 or pSB5.1.7.1)
• purpose: high copy number plasmid to be used in the three antibiotic selection assembly technique.
• derived from pSB1A3-1
• constructed by Reshma

pSB1AC3-P1010

• (formerly known as pSB1AC3-1)
• purpose: high copy number plasmid to be used in the three antibiotic selection assembly technique.
• derived from pSB1A3-1
• constructed by Robert Bryant

pSB2* Plasmids

pSB2K0

• (also known as pSCANS)
• purpose: inducible copy number plasmid
• problems: contains a PstI site in backbone

pSB2K3-P1010

• (formerly known as pSB2K3-1)
• purpose: IPTG-inducible copy number plasmid
• derived from pSB2K0
• constructed by Sri Kosuri

pSB2K4-P1010

• (formerly known as pSB2K4-1)
• purpose: remove many restriction sites from backbone of pSB2K
• Created by a backport from a BioBricks++ plasmid back to a BioBricks cloning site
• Contains terminators on both sides of the cloning site, but also contains two extra restriction sites around the cloning site due to process of construction. Does not affect BioBricks construction in any way.
• Has approximately 2 dozen point mutations in the backbone relative to pSB2K3
• Derived from pREB1.3 from Leon who removed many of the symmetric restriction sites
• Further mutations were done to make backbone clean for some Type IIs restriction enzymes including: AarI, AcuI, BbsI, BbvCI, BciVI, BsaI, BsmBI, EarI, N.BstNBI, SgrAI
• Plasmid is still inducible and functions similarly to pSB2K3
• constructed by Austin

pSB3* Plasmids

pSB3K1

• (formerly known as p15A-Kan v2.0)
• purpose: low copy number plasmid to be used for unstable assemblies that contain lac components.
• constructed by Reshma

pSB3K3-P1010

• (formerly known as pSB3K3-1)
• purpose: low copy number plasmid to be used for unstable assemblies that contain lac components. Contains ccdB to facilitate cloning.
• derived from pSB3K1
• constructed by Reshma

pSB4* Plasmids

pSB4A3-P1010

• (formerly known as pSB4A3-1)
• purpose: low copy number plasmid to be used for unstable assemblies that contain lac components. Contains ccdB to facilitate cloning.
• derived from pSB4A1
• constructed by Reshma

pSB4A1

• (formerly known as pSC101 v1.1)
• purpose: low copy number plasmid to be used for unstable assemblies that contain lac components.
• derived from Elowitz's repressilator plasmid
• constructed by Reshma