Synthetic Biology:Vectors/Parts: Difference between revisions

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==Replication origins==
==Replication origins==
*[http://parts2.mit.edu/r/parts/partsdb/view.cgi?part_id=6307 BBa_I50000]: F plasmid backbone with BioBricks restriction sites removed
*<bbpart>BBa_I50000</bbpart>: F plasmid backbone with BioBricks restriction sites removed
*[http://parts2.mit.edu/r/parts/partsdb/view.cgi?part_id=6336 BBa_I50010]: oriV origin which requires TrfA protein to be functional.
*<bbpart>BBa_I50010</bbpart>: oriV origin which requires TrfA protein to be functional.
*[http://parts.mit.edu/r/parts/partsdb/view.cgi?part_id=6400 BBa_I50020]: high copy origin of replication from pSB1A3
*<bbpart>BBa_I50020</bbpart>: high copy origin of replication from pSB1A3


==Antibiotic resistance cassettes==
==Antibiotic resistance cassettes==
*[http://parts2.mit.edu/r/parts/partsdb/view.cgi?part_id=6370 BBa_P1001]: cassette providing tetracycline resistance.
*<bbpart>BBa_P1001</bbpart>: cassette providing tetracycline resistance.
*[http://parts2.mit.edu/r/parts/partsdb/view.cgi?part_id=6369 BBa_P1000]: cassette providing chloramphenicol resistance.
*<bbpart>BBa_P1000</bbpart>: cassette providing chloramphenicol resistance.
*[http://parts.mit.edu/r/parts/partsdb/view.cgi?part_id=6440 BBa_P1002]: cassette providing ampicillin resistance.
*<bbpart>BBa_P1002</bbpart>: cassette providing ampicillin resistance.
*additional antibiotic resistance cassettes (from Tom)
*<bbpart>BBa_P1003</bbpart>: cassette providing kanamycin resistance.


==Terminators==
==Terminators==
*[http://parts2.mit.edu/r/parts/partsdb/view.cgi?part_id=6306 BBa_B0055]: upstream flanking terminator
*<bbpart>BBa_B0055</bbpart>: upstream flanking terminator
*[http://parts2.mit.edu/r/parts/partsdb/view.cgi?part_id=6305 BBa_B0054]: downstream flanking terminator
*<bbpart>BBa_B0054</bbpart>: downstream flanking terminator


===Notes===
===Notes===
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==Others==
==Others==
*[http://parts.mit.edu/r/parts/partsdb/view.cgi?part_id=6396 BBa_I1000] (old) or [http://parts.mit.edu/r/parts/partsdb/view.cgi?part_id=4941 BBa_P1010] (preferred): ccd operon in BioBricks format
*<bbpart> BBa_I1000</bbpart> (old) or <bbpart>BBa_P1010</bbpart> (preferred): ccd operon in BioBricks format
*unique identifier for the vector
*unique identifier for the vector

Revision as of 10:54, 23 January 2006

This is a list of BioBricks parts for use in construction of modular vectors.

Some have just been designed while others have been constructed and tested. See the associated registry page for details.

Replication origins

  • <bbpart>BBa_I50000</bbpart>: F plasmid backbone with BioBricks restriction sites removed
  • <bbpart>BBa_I50010</bbpart>: oriV origin which requires TrfA protein to be functional.
  • <bbpart>BBa_I50020</bbpart>: high copy origin of replication from pSB1A3

Antibiotic resistance cassettes

  • <bbpart>BBa_P1001</bbpart>: cassette providing tetracycline resistance.
  • <bbpart>BBa_P1000</bbpart>: cassette providing chloramphenicol resistance.
  • <bbpart>BBa_P1002</bbpart>: cassette providing ampicillin resistance.
  • <bbpart>BBa_P1003</bbpart>: cassette providing kanamycin resistance.

Terminators

  • <bbpart>BBa_B0055</bbpart>: upstream flanking terminator
  • <bbpart>BBa_B0054</bbpart>: downstream flanking terminator

Notes

It wasn't clear from the website if these were bi-directional? Not sure that this is very important--BC

I don't know if the terminators are bidirectional. These terminators are used as flanking terminators in other vectors and are claimed to make the cloning of difficult pieces of DNA (like strong promoters) easier. This is why I was planning on orienting the antibiotic resistance cassette in the opposite direction so that read through from upstream of the multiple cloning site is less of an issue. -- RS

Others

  • <bbpart> BBa_I1000</bbpart> (old) or <bbpart>BBa_P1010</bbpart> (preferred): ccd operon in BioBricks format
  • unique identifier for the vector
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