Synthetic Biology:Vectors/Wishlist: Difference between revisions
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purpose: inducible copy number plasmid to be used in the three antibiotic selection | purpose: inducible copy number plasmid to be used in the three antibiotic selection | ||
assembly technique. | assembly technique. | ||
==pSB4*== | |||
Randy would like to have the full complement of low copy vectors for assembly purposes. | |||
Here is what they might look like using a pSC101 origin: | |||
<b> | |||
5' BBa_P1010 (ccd) -- | |||
BBsuffix -- [[Topoisomerase I mediated TA cloning | TOPO site]] -- translational stop sequence -- [[Synthetic Biology:Vectors/Barcode | plasmid barcode]] -- BBa_B0054 (terminator) -- BBa_G00102 (VR) -- BBa_I50040 (pSC101) -- BBa_P1005 or BBa_P1006 or BBa_P1007 (TetR or CmR or KanR with AmpR) -- | |||
BBa_G00100 (VF2) -- BBa_B0055 (terminator) -- [[Synthetic Biology:Vectors/Barcode | plasmid barcode]] -- translational stop sequence -- [[Topoisomerase I mediated TA cloning | TOPO site]] -- BB prefix 3' | |||
</b> | |||
==pSB5*== | ==pSB5*== | ||
A [[Synthetic Biology:Vectors/Single copy plasmid | single copy plasmid]] for characterization and operation of devices and systems. | A [[Synthetic Biology:Vectors/Single copy plasmid | single copy plasmid]] for characterization and operation of devices and systems. | ||
==pSB6*== | |||
Randy would like to have the full complement of low copy vectors for assembly purposes. | |||
Here is what they might look like using a pBR322 origin: | |||
<b> | |||
5' BBa_P1010 (ccd) -- | |||
BBsuffix -- [[Topoisomerase I mediated TA cloning | TOPO site]] -- translational stop sequence -- [[Synthetic Biology:Vectors/Barcode | plasmid barcode]] -- BBa_B0054 (terminator) -- BBa_G00102 (VR) -- BBa_I50030 (pSC101) -- BBa_P1005 or BBa_P1006 or BBa_P1007 (TetR or CmR or KanR with AmpR) -- | |||
BBa_G00100 (VF2) -- BBa_B0055 (terminator) -- [[Synthetic Biology:Vectors/Barcode | plasmid barcode]] -- translational stop sequence -- [[Topoisomerase I mediated TA cloning | TOPO site]] -- BB prefix 3' | |||
</b> | |||
Revision as of 16:01, 3 February 2006
This is a wishlist of vectors that we would like to have but are not currently available.
pSB2*
pSB2AT3-P1010
purpose: inducible copy number plasmid to be used in the three antibiotic selection assembly technique.
pSB2AK3-P1010
purpose: inducible copy number plasmid to be used in the three antibiotic selection assembly technique.
pSB2AC3-P1010
purpose: inducible copy number plasmid to be used in the three antibiotic selection assembly technique.
pSB4*
Randy would like to have the full complement of low copy vectors for assembly purposes.
Here is what they might look like using a pSC101 origin:
5' BBa_P1010 (ccd) --
BBsuffix -- TOPO site -- translational stop sequence -- plasmid barcode -- BBa_B0054 (terminator) -- BBa_G00102 (VR) -- BBa_I50040 (pSC101) -- BBa_P1005 or BBa_P1006 or BBa_P1007 (TetR or CmR or KanR with AmpR) --
BBa_G00100 (VF2) -- BBa_B0055 (terminator) -- plasmid barcode -- translational stop sequence -- TOPO site -- BB prefix 3'
pSB5*
A single copy plasmid for characterization and operation of devices and systems.
pSB6*
Randy would like to have the full complement of low copy vectors for assembly purposes.
Here is what they might look like using a pBR322 origin:
5' BBa_P1010 (ccd) --
BBsuffix -- TOPO site -- translational stop sequence -- plasmid barcode -- BBa_B0054 (terminator) -- BBa_G00102 (VR) -- BBa_I50030 (pSC101) -- BBa_P1005 or BBa_P1006 or BBa_P1007 (TetR or CmR or KanR with AmpR) --
BBa_G00100 (VF2) -- BBa_B0055 (terminator) -- plasmid barcode -- translational stop sequence -- TOPO site -- BB prefix 3'
