Synthetic Biology:Vectors/Wishlist
This is a wishlist of vectors that we would like to have but are not currently available.
pSB2*
pSB2AT3-P1010
purpose: inducible copy number plasmid to be used in the three antibiotic selection assembly technique.
pSB2AK3-P1010
purpose: inducible copy number plasmid to be used in the three antibiotic selection assembly technique.
pSB2AC3-P1010
purpose: inducible copy number plasmid to be used in the three antibiotic selection assembly technique.
pSB4*
Randy would like to have the full complement of low copy vectors for assembly purposes.
Here is what they might look like using a pSC101 origin:
5' BBa_P1010 (ccd) --
BBa_G00001 (BB suffix) -- BBa_B0044 (TOPO site) -- BBa_B0042 (translational stop sequence) -- plasmid barcode -- BBa_B0054 (terminator) -- BBa_G00102 (VR) -- BBa_P1005 or BBa_P1006 or BBa_P1007 (CmR or TetR or KanR with AmpR) -- BBa_I50041 (pSC101, reverse) --
BBa_G00100 (VF2) -- BBa_B0055 (terminator) -- plasmid barcode -- BBa_B0042 (translational stop sequence) -- BBa_B0043 (TOPO site) -- BBa_G00000 (BB prefix) 3'
Total length (excluding scars and barcode, including BioBricks prefix and suffix)
- CmR version: 5013bp
- TetR version: 5503bp
- KanR version: 5217bp
Total length (excluding barcode, including BioBricks prefix, suffix and scars)
- CmR version: 5103bp
- TetR version: 5593bp
- KanR version: 5307bp
pSB5*
A single copy plasmid for characterization and operation of devices and systems.
5' BBa_P1010 (ccd) -- BBa_I50020 (hc ori)--
BBa_G00001 (BB suffix) -- BBa_B0044 (TOPO site) -- BBa_B0042 (translational stop sequence) -- plasmid barcode -- BBa_B0054 (terminator) -- BBa_G00102 (VR) -- BBa_P1000 or BBa_P1001 or BBa_P1003 (CmR or TetR or KanR) -- BBa_B0053 (His terminator) -- BBa_P1002 (AmpR) -- BBa_B0062 (rrnC terminator) -- BBa_I50001 (F plasmid, reverse)
BBa_G00100 (VF2) -- BBa_B0055 (terminator) -- plasmid barcode -- BBa_B0042 (translational stop sequence) -- BBa_B0043 (TOPO site) -- BBa_G00000 (BB prefix) 3'
Total length (excluding scars and barcode, including prefix and suffix)
- CmR version: 8296bp
- TetR version: 8786bp
- KanR version: 8500bp
Total length (excluding barcode, including prefix, suffix and barcodes)
- CmR version: 8392bp
- TetR version: 8882bp
- KanR version: 8596bp
pSB6*
Randy would like to have the full complement of low copy vectors for assembly purposes.
Here is what they might look like using a pBR322 origin:
5' BBa_P1010 (ccd) --
BBa_G00001 (BB suffix) -- BBa_B0044 (TOPO site) -- BBa_B0042 (translational stop sequence) -- plasmid barcode -- BBa_B0054 (terminator) -- BBa_G00102 (VR) -- BBa_P1005 or BBa_P1006 or BBa_P1007 (CmR or TetR or KanR with AmpR) -- BBa_I50030 (pBR322, switch orientation) --
BBa_G00100 (VF2) -- BBa_B0055 (terminator) -- plasmid barcode -- BBa_B0042 (translational stop sequence) -- BBa_B0043 (TOPO site) -- BBa_G00000 (BB prefix) 3'
Total length (excluding scars and barcode, including BioBricks prefix and suffix)
- CmR version: 4814bp
- TetR version: 5304bp
- KanR version: 5018bp
Total length (excluding barcode, including BioBricks prefix, suffix and scars)
- CmR version: 4904bp
- TetR version: 5394bp
- KanR version: 5108bp
