Synthetic Biology Standardization Debate
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==== Microscopy Standardization ==== | ==== Microscopy Standardization ==== | ||
| - | + | # what reporters are more suitable for bulk measure? or single-cell? or flow cytometry? | |
| - | + | # how reporter half-life, maturation time, brightness, predisposition to form aggregates affect measurements? | |
| + | # what about multicolor imaging? how we solve the close emission spectra of the different red-emitting reporters? | ||
| + | # what about degradation tags, can they turn out to be useful for programmed and precise control of reporters half-life? | ||
=== Standardization of Gene Expression === | === Standardization of Gene Expression === | ||
Current revision
This page collects thoughts and materials for a debate between the Arkin and Endy labs about standardization in Synthetic Biology.
Contents |
Ideas to Debate
Standardization of Measurement
Microscopy Standardization
- what reporters are more suitable for bulk measure? or single-cell? or flow cytometry?
- how reporter half-life, maturation time, brightness, predisposition to form aggregates affect measurements?
- what about multicolor imaging? how we solve the close emission spectra of the different red-emitting reporters?
- what about degradation tags, can they turn out to be useful for programmed and precise control of reporters half-life?
Standardization of Gene Expression
Transcriptional Standardization
- Can this be standardized?
Translational Standardization
- Can this be standardized?
Transcript Degradation Standardization
- Can this be standardized?
Protein Degradation Standardization
- Can this be standardized?
Standardization of 'Format'
Plasmid Standardization
- Which origins?
- Do we have data on plasmid localization?
Genome Insertion Standardization
- Do we have a standard locus for insertion?
Strain Standardization
- Should we be working in the same genetic background?
