Synthetic Society/SB Reality
From OpenWetWare
Construction Technology
Conclusion: It is possible to construct any piece of DNA.
Characteristics as of 1 February 2006:
- Cost. ~$1 per bp
- Length. ~100 bp direct chemical synthesis, ~15,000 bp PCR assembly, ~3,500,000 bp inchworm elongation
- Error Rate. From 1:10 to effectively zero
- Time. 6 hours for 100 bp, 2-6 weeks for 15,000 bp, ~2 months for 50,000 bp, ~7 years for 3,500,000 bp
- Diversity. From one molecule to millions of molecules (heavily dependent on method)
- Terms of Sale. Varied (from no-strings-attached to full-rights-reserved).
Examples:
- 1918 Flu resurrection
- Refactoring Bacteriophage T7
- Poliovirus synthesis
- PhiX174 synthesis
- E.coli camera
- Programmed pattern formation
- Venter at NSABB
- Two genomes, one cell
- Stemmer
- Baric
Ownership, Sharing, & Innovation Technology
- MTA examples
- BioBrick examples
