Sysbio:Back Door: Difference between revisions
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# Keep your bench as clean and organize as possible. | # Keep your bench as clean and organize as possible. | ||
#Label everything. Keep track of all the solutions you prepare. Labeling basics are: Name, Initials of owner, date of preparation, concentration when possible. | |||
#Do not do anything that you are not completely sure about what you are doing. Ask for help. | #Do not do anything that you are not completely sure about what you are doing. Ask for help. | ||
# Always wear gloves, unless you are working with the flame. | # Always wear gloves, unless you are working with the flame. | ||
# You generally do not need a lab coat. If you really need one, ask *'''[[User:DavidMiguez|David]] | # You generally do not need a lab coat. If you really need one, ask *'''[[User:DavidMiguez|David]]'''. | ||
# When working with UV light (DNA gels...), avoid direct eye exposure. Put the orange googles before turning on the light. | # When working with UV light (DNA gels...), avoid direct eye exposure. Put the orange googles before turning on the light. | ||
# The lab do not generally uses EtBr for DNA staining, if you need to do so, label everything you use as EtBr positive and ask *'''[[User:DavidMiguez|David]] | # The lab do not generally uses EtBr for DNA staining, if you need to do so, label everything you use as EtBr positive and ask *'''[[User:DavidMiguez|David]] ''' for storage. Avoid direct exposure of your skin and be very cautious. | ||
# When managing sharp razors, broken glass or needles, dispose them in the special yellow container. | # When managing sharp razors, broken glass or needles, dispose them in the special yellow container. | ||
# When one of the containers is full, call waste management "620 012 703" (Ricardo) | # When one of the containers is full, call waste management "620 012 703" (Ricardo) | ||
# As you know, safety conditions here at | # As you know, safety conditions here at the INC are not optimal (no eye shower, no safety shower...). At this point, it is very little what we can do to improve that, so please be extra careful when managing acids or corrosive chemicals. Use the hood in the chemical room, and prepare all your solutions there. | ||
====Tissue Culture==== | ====Tissue Culture==== | ||
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===Protocols=== | ===Protocols=== | ||
All protocols at this point can be found at the lab google drive. Ask for an invitation. | |||
===Meetings, Seminars, Deadlines, Birthdays...=== | ===Meetings, Seminars, Deadlines, Birthdays...=== | ||
<html><iframe src="https://www.google.com/calendar/embed?height= | <html><iframe src="https://www.google.com/calendar/embed?height=800&wkst=2&bgcolor=%23FFFFFF&src=njji34mpd0eq577495eedal070%40group.calendar.google.com&color=%232952A3&src=spain__en%40holiday.calendar.google.com&color=%234E5D6C&ctz=Europe%2FMadrid" style=" border-width:0 " width="1015" height="800" frameborder="0" scrolling="no"></iframe></iframe></html> | ||
== | * Subscribe to '''Lab Schedule''' in [https://www.google.com/calendar/feeds/njji34mpd0eq577495eedal070%40group.calendar.google.com/public/basic XML], [https://www.google.com/calendar/ical/njji34mpd0eq577495eedal070%40group.calendar.google.com/public/basic.ics iCal], [https://www.google.com/calendar/embed?src=njji34mpd0eq577495eedal070%40group.calendar.google.com&ctz=Europe/Madrid HTML] | ||
===Lab Pictures=== | |||
{|class="white" | {|class="white" | ||
|width="1024px" class="" cellpadding="0"| {{navbar_icons}} | |width="1024px" class="" cellpadding="0"| {{navbar_icons}} |
Revision as of 07:27, 29 August 2013
Home Contact Lab Members Publications Research Teaching Internal Lab Notebook Lab Blog
Lab safetyGeneral Lab Rules
Tissue CultureThis protocol describes techniques to be used for tissue culture in the SysBio lab: These guidelines must be followed!- failure to do so can result in damage to your and the experiments of others. To protect the overall lab, you need first to be trained by a certified Tissue Culture Master before beginning work. Only a Tissue Culture Master can certify you as a Tissue Culture Master that can train others, so be nice to them. Tissue Culture Masters: - Adriana Sanz Space: You will be assigned a space in a tissue culture incubator. You will also be assigned a space at the refrigerator (4 C) and at the freezer (-20 C). You must use the hoods and incubators only in the room you were assigned. If you need space in the Criofreezer (-80 C) container for archival of cell lines, contact David G. Miguez. Safety: Although most cell lines are reasonably safe to work with, be aware that you are working with or around potentially dangerous materials To protect everyone’s safety, follow these guidelines:
Aseptic Technique: Contamination by microorganisms is a major problem in tissue culture. Bacteria, mycoplasma, yeast, and fungal spores may be introduced via the operator, the atmosphere, work surfaces, solutions and many other sources. Proper aseptic technique seeks to eliminate these contaminants. It is important to establish and maintain aseptic techniques particularly given the importance of cell culture to everyone’s experiments in the lab. Contamination can be minor and confined to one or two cultures, can spread among several cultures and infect a whole experiment, or can be widespread and wipe out your entire stock. Catastrophes can be minimized if :
All materials that will come into direct contact with the culture must be sterile. Aseptic technique is a combination of procedures designed to reduce the probability of infection. Most cell culture work is done in a laminar flow hood. General Rules:
5) Remove liquid from containers at an angle. 6) Clean with 70% ethanol often (surfaces and gloves). 7) Remember that it is the inside of containers (autoclaved bottles, centrifuge tubes, etc) that is sterile.. treat the outside as dirty and clean with EtOH. Start-up in the hood : The major advantage of working in a laminar flow hood is that the working environment is protected from contamination by a constant flow of filtered air. A vertical flow hood is where air blows down from the top of the hood onto the work surface. Follow these guidelines when using the laminar flow hood. Keep this direction of flow in mind when setting up your work area.
Working in the Hood:
Using the UV Light: Although not recommended for routine sterilization of the hood itself, each hood has a UV light which can be used to sterilize surfaces, etc.
Incubators and Microscope: These are shared equipment, so they present a great method to spread contamination!
When You Are Finished
ProtocolsAll protocols at this point can be found at the lab google drive. Ask for an invitation. Meetings, Seminars, Deadlines, Birthdays...<html><iframe src="https://www.google.com/calendar/embed?height=800&wkst=2&bgcolor=%23FFFFFF&src=njji34mpd0eq577495eedal070%40group.calendar.google.com&color=%232952A3&src=spain__en%40holiday.calendar.google.com&color=%234E5D6C&ctz=Europe%2FMadrid" style=" border-width:0 " width="1015" height="800" frameborder="0" scrolling="no"></iframe></iframe></html>
Lab Pictures
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