TAE
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TAE is a commonly used buffer for making and running DNA agarose gels. | TAE is a commonly used buffer for making and running DNA agarose gels. | ||
It offers a few advantages and disadvantages compared to TBE buffer: | It offers a few advantages and disadvantages compared to TBE buffer: | ||
| + | *TAE buffer provides optimal resolution of fragments >4 kb in length, while TBE provides better resolution for 0.1 to 3 kb fragments. | ||
* TAE is significantly cheaper to make | * TAE is significantly cheaper to make | ||
* TAE stocks can be 50X concentrated and therefore take up less space than 10X concentrated TBE stock | * TAE stocks can be 50X concentrated and therefore take up less space than 10X concentrated TBE stock | ||
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To make 1x TAE from 50X TAE stock, dilute 20ml of stock into 980 ml of deionised water. | To make 1x TAE from 50X TAE stock, dilute 20ml of stock into 980 ml of deionised water. | ||
| + | ==See also== | ||
| - | [[Category:Material]] | + | For disambiguation purposes, see [[EDTA]] and [[TE]]. |
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| + | [[Category:Material]] | ||
| + | [[Category:Agarose gel electrophoresis]] | ||
Revision as of 09:29, 13 February 2013
TAE is a commonly used buffer for making and running DNA agarose gels. It offers a few advantages and disadvantages compared to TBE buffer:
- TAE buffer provides optimal resolution of fragments >4 kb in length, while TBE provides better resolution for 0.1 to 3 kb fragments.
- TAE is significantly cheaper to make
- TAE stocks can be 50X concentrated and therefore take up less space than 10X concentrated TBE stock
- TBE offers a higher resolution and has a higher buffering capacity at greater temperatures induced by relatively higher voltages
- TBE can negatively influence the yield of DNA after recovery from a gel when using glass-based protocols. Yield does not seem to be influenced when using silica-based methods.
- The bromide ion in TBE is a powerful inhibitor of enzyme activity. This can prevent degradation of nucleic acid, but can also interfere with subsequent experiments like cloning DNA into a vector.
Ingredients for one litre 50X stock
Add dH2O up to one litre.
To make 1x TAE from 50X TAE stock, dilute 20ml of stock into 980 ml of deionised water.
