TBE

Purpose

• TBE or Tris/Borate/EDTA is used for electrophoresis of nucleic acids in sequencing PAA gels or agarose gels.

Recipe

10x TBE (1 liter):

1. Dissolve 108 g Tris and 55 g Boric acid in 900 ml distilled water.
2. Add 40 ml 0.5 M Na₂EDTA (pH 8.0) (alternatively use 9.3 g Na₂EDTA)
3. Adjust volume to 1 Liter.
4. Store at room temperature.

Note: 10x TBE may take some time to dissolve, even with fast stirring

1x TBE (1 liter):

1. Dissolve 10.8 g Tris and 5.5 g Boric acid in 900 ml distilled water.
2. Add 4 ml 0.5 M Na₂EDTA (pH 8.0)
3. Adjust volume to 1 Liter.
4. Store at room temperature.

Commercial sources

1. UltraPure™ 10X TBE Buffer from Invitrogen