TBE
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Purpose
- TBE is used for electrophoresis of nucleic acids in sequencing PAA gels or agarose gels.
Recipe
10x TBE (1 liter):
- Dissolve 108 g Tris and 55 g Boric acid in 900 ml distilled water.
- Add 40 ml 0.5 M Na2EDTA (pH 8.0) (alternatively use 9.3 g Na2EDTA)
- Adjust volume to 1 Liter.
- Store at room temperature.
1x TBE (1 liter):
- Dissolve 10.8 g Tris and 5.5 g Boric acid in 900 ml distilled water.
- Add 4 ml 0.5 M Na2EDTA (pH 8.0)
- Adjust volume to 1 Liter.
- Store at room temperature.
Commercial sources
- UltraPure™ 10X TBE Buffer from Invitrogen