TBS: Difference between revisions
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*Prepare and sterile filter. Store at 4°C. After diluting the 5x to 1x filter again | *Prepare and sterile filter. Store at 4°C. After diluting the 5x to 1x filter again | ||
*'''[[TBST]]''': For some applications one adds Tween 20 or | *'''[[TBST]]''': For some applications one adds Tween 20 or Triton X-100 to TBS which makes TBST (people use the name TBST for both, probably it's lab dependent. | ||
**Usual concentrations of Tween 20 in TBST are 0.1 or 0.05%. | **Usual concentrations of Tween 20 in TBST are 0.1 or 0.05%. | ||
**Usual concentration of | **Usual concentration of Triton X-100 in TBST is 0.1%. | ||
Revision as of 07:42, 12 January 2009
Purpose
- TBS stands for tris-buffered saline and is used in many protocols for washing
Recipe
- Endconzentration in 1x TBS will be 20mM Tris and 150mM NaCl
| for 1 liter with stock solutions |
|---|
| 100 ml 1M Tris-HCl pH 7.5 |
| 150 ml 5M NaCl |
| 750 ml ddH2O |
- Prepare and sterile filter. Store at 4°C. After diluting the 5x to 1x filter again
- TBST: For some applications one adds Tween 20 or Triton X-100 to TBS which makes TBST (people use the name TBST for both, probably it's lab dependent.
- Usual concentrations of Tween 20 in TBST are 0.1 or 0.05%.
- Usual concentration of Triton X-100 in TBST is 0.1%.
